Linked Life Data

12234014

Source:http://linkedlifedata.com/resource/pubmed/id/12234014

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2002-9-17
pubmed:abstractText
The occurrence of extended tight junction (TJ) structures, including zonulae occludentes (ZO), and the spatial arrangement of TJ proteins in stratified mammalian epithelia has long been controversially discussed. Therefore, we have systematically examined the localization of TJ proteins in diverse stratified epithelial tissues (e.g., epidermis, heel pad, snout, gingiva, tongue, esophagus, exocervix, vagina, urothelium, cornea) of various species (human, bovine, rodents) as well as in human cell culture lines derived from stratified epithelia, by electron microscopy as well as by immunocytochemistry at both the light and the electron microscopic level, using antibodies to TJ proteins such as occludin, claudins 1 and 4, protein ZO-1, cingulin and symplekin. We have found an unexpected diversity of TJ-related structures of which only those showing colocalization with the most restricted transmembrane TJ marker protein, occludin, are presented here. While in epidermis and urothelium occludin is restricted to the uppermost living cell layer, TJ-related junctions are abundant in the upper third or even in the majority of the suprabasal cell layers in other stratified epithelia. Interfollicular epidermis contains, in the stratum granulosum, extended, probably continuous ZO-like structures which can also be traced at least through the Henle cell layer of hair follicles. Similar apical ZO-like structures have been seen in the upper living cell layers of all other stratified epithelia and cell cultures examined, but in most of them we have noticed, in addition, junctional regions showing relatively broad, ribbon-like membrane contacts which in cross-section often appear pentalaminar, with an electron-dense middle lamella ("lamellated TJs", coniunctiones laminosae). In suprabasal layers of several stratified epithelia we have further observed TJ protein-containing junctions of variable sizes which are characterized by a 10-30-nm dense lamina interposed between the two membranes ("sandwich junctions"; iuncturae structae). Moreover, we have often observed variously sized regions in which the intermembrane distance is rather regularly bridged by short rod-like elements ("cross-bridged cell walls"; parietes transtillati), often in close vicinity of TJ-related structures or desmosomes. The significance of these structures and their possible biological importance are discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0171-9335
pubmed:author
pubmed:issnType
Print
pubmed:volume
81
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
419-35
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12234014-Adult, pubmed-meshheading:12234014-Animals, pubmed-meshheading:12234014-Cattle, pubmed-meshheading:12234014-Cell Adhesion, pubmed-meshheading:12234014-Cell Communication, pubmed-meshheading:12234014-Epithelial Cells, pubmed-meshheading:12234014-Epithelium, pubmed-meshheading:12234014-Female, pubmed-meshheading:12234014-Fetus, pubmed-meshheading:12234014-Fluorescent Antibody Technique, pubmed-meshheading:12234014-Humans, pubmed-meshheading:12234014-Male, pubmed-meshheading:12234014-Membrane Proteins, pubmed-meshheading:12234014-Microfilament Proteins, pubmed-meshheading:12234014-Microscopy, Electron, pubmed-meshheading:12234014-Nuclear Proteins, pubmed-meshheading:12234014-Phosphoproteins, pubmed-meshheading:12234014-Pregnancy, pubmed-meshheading:12234014-Proteins, pubmed-meshheading:12234014-Tight Junctions, pubmed-meshheading:12234014-Tumor Cells, Cultured
pubmed:year
2002
pubmed:articleTitle
Tight junctions and compositionally related junctional structures in mammalian stratified epithelia and cell cultures derived therefrom.
pubmed:affiliation
Division of Cell Biology, German Cancer Research Center, Heidelberg.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't