Linked Life Data

12231255

Source:http://linkedlifedata.com/resource/pubmed/id/12231255

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2002-9-16
pubmed:abstractText
The present study is focused on biosynthesis of adrenal steroids in the frog brain. Employing RT-PCR method using total RNA from the adult Rana nigromaculata brain, we isolated a 419-bp fragment of cDNA encoding cytochrome P450(11beta,aldo), which catalyzes the final step of biosynthesis of the frog adrenal steroids, corticosterone and aldosterone. The deduced amino acid sequence of R. nigromaculata brain cytochrome P450(11beta,aldo) shared a high homology (88.8%) with that of R. catesbeiana adrenal cytochrome P450(11beta,aldo). Southern blot analysis of the RT-PCR product confirmed the P450(11beta,aldo) transcription in the frog brain without a clear-cut sex difference. Then, we analyzed the P450(11beta,aldo) mRNA expression in different brain regions of the adult frog by RT-PCR method. The P450(11beta,aldo) gene was transcribed in the telencephalon, diencephalon, midbrain, and cerebellum. The transcript level of the frog beta-actin gene was relatively constant in all the frog samples examined. In situ hybridization analysis showed that the P450(11beta,aldo) gene was transcribed abundantly in the cells throughout the frog brain, such as the pallium mediale in the telencephalon, the nucleus preopticus in the diencephalon, the stratum griseum superficiale tecti in the midbrain, and Purkinje cells in the cerebellum. These results taken together suggest that the frog brain synthesizes adrenal steroids, such as corticosterone and aldosterone.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
950
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
288-96
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Expression and localization of cytochrome P450(11beta,aldo) mRNA in the frog brain.
pubmed:affiliation
Institute for Amphibian Biology, Graduate School of Science, Hiroshima University, 739-8526, Higashi-Hiroshima, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't