| pubmed-article:12228313 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12228313 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:12228313 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:12228313 | lifeskim:mentions | umls-concept:C1660642 | lld:lifeskim |
| pubmed-article:12228313 | lifeskim:mentions | umls-concept:C0004573 | lld:lifeskim |
| pubmed-article:12228313 | lifeskim:mentions | umls-concept:C0444659 | lld:lifeskim |
| pubmed-article:12228313 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:12228313 | pubmed:dateCreated | 2002-9-13 | lld:pubmed |
| pubmed-article:12228313 | pubmed:abstractText | The cellular localization of Babesia bovis rhoptry-associated protein 1 (RAP-1) and its erythrocyte-binding affinity were examined with anti-RAP-1 antibodies. In an indirect immunofluorescent antibody test, RAP-1 was detectable in all developmental stages of merozoites and in extracellular merozoites. In the early stage of merozoite development, RAP-1 appears as a dense accumulation, which later thins out and blankets the host cell cytoplasm, but retains a denser mass around newly formed parasite nuclei. The preferential accumulations of RAP-1 on the inner surface of a host cell membrane and bordering the parasite's outer surface were demonstrable by immunoelectron microscopy. An erythrocyte-binding assay with the lysate of merozoites demonstrated RAP-1 binding to both bovine and equine erythrocytes. Anti-RAP-1 monoclonal antibody 1C1 prevented the interaction of RAP-1 with bovine erythrocytes and significantly inhibited parasite proliferation in vitro. With the recombinant RAP-1, the addition of increasing concentrations of Ca(2+) accentuated its binding affinity with bovine erythrocytes. The present findings lend support to an earlier proposition of an erythrocytic binding role for RAP-1 expressed in B. bovis merozoites and, possibly, its involvement in the escape of newly formed merozoites from host cells. | lld:pubmed |
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| pubmed-article:12228313 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:12228313 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:12228313 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12228313 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:12228313 | pubmed:issn | 0019-9567 | lld:pubmed |
| pubmed-article:12228313 | pubmed:author | pubmed-author:SugimotoChihi... | lld:pubmed |
| pubmed-article:12228313 | pubmed:author | pubmed-author:InoueNoboruN | lld:pubmed |
| pubmed-article:12228313 | pubmed:author | pubmed-author:HirataHaruyuk... | lld:pubmed |
| pubmed-article:12228313 | pubmed:author | pubmed-author:YokoyamaNaoak... | lld:pubmed |
| pubmed-article:12228313 | pubmed:author | pubmed-author:IgarashiIkuoI | lld:pubmed |
| pubmed-article:12228313 | pubmed:author | pubmed-author:SuthisakBoonc... | lld:pubmed |
| pubmed-article:12228313 | pubmed:author | pubmed-author:MatsuoTomohid... | lld:pubmed |
| pubmed-article:12228313 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12228313 | pubmed:volume | 70 | lld:pubmed |
| pubmed-article:12228313 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12228313 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12228313 | pubmed:pagination | 5822-6 | lld:pubmed |
| pubmed-article:12228313 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:12228313 | pubmed:meshHeading | pubmed-meshheading:12228313... | lld:pubmed |
| pubmed-article:12228313 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12228313 | pubmed:articleTitle | Cellular localization of Babesia bovis merozoite rhoptry-associated protein 1 and its erythrocyte-binding activity. | lld:pubmed |
| pubmed-article:12228313 | pubmed:affiliation | National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan. | lld:pubmed |
| pubmed-article:12228313 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12228313 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:12228313 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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