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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2002-9-13
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pubmed:abstractText |
The cellular localization of Babesia bovis rhoptry-associated protein 1 (RAP-1) and its erythrocyte-binding affinity were examined with anti-RAP-1 antibodies. In an indirect immunofluorescent antibody test, RAP-1 was detectable in all developmental stages of merozoites and in extracellular merozoites. In the early stage of merozoite development, RAP-1 appears as a dense accumulation, which later thins out and blankets the host cell cytoplasm, but retains a denser mass around newly formed parasite nuclei. The preferential accumulations of RAP-1 on the inner surface of a host cell membrane and bordering the parasite's outer surface were demonstrable by immunoelectron microscopy. An erythrocyte-binding assay with the lysate of merozoites demonstrated RAP-1 binding to both bovine and equine erythrocytes. Anti-RAP-1 monoclonal antibody 1C1 prevented the interaction of RAP-1 with bovine erythrocytes and significantly inhibited parasite proliferation in vitro. With the recombinant RAP-1, the addition of increasing concentrations of Ca(2+) accentuated its binding affinity with bovine erythrocytes. The present findings lend support to an earlier proposition of an erythrocytic binding role for RAP-1 expressed in B. bovis merozoites and, possibly, its involvement in the escape of newly formed merozoites from host cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12228313-10580049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12228313-10885987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12228313-10964646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12228313-11083806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12228313-11730795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12228313-11854249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12228313-1441189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12228313-15275182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12228313-1715329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12228313-1741019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12228313-2473398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12228313-3410541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12228313-3905658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12228313-7312033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12228313-7355284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12228313-7360274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12228313-7687587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12228313-8100672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12228313-8433711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12228313-9650921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12228313-9662706
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
70
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5822-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12228313-Animals,
pubmed-meshheading:12228313-Antibodies, Monoclonal,
pubmed-meshheading:12228313-Antibodies, Protozoan,
pubmed-meshheading:12228313-Babesia bovis,
pubmed-meshheading:12228313-Babesiosis,
pubmed-meshheading:12228313-Base Sequence,
pubmed-meshheading:12228313-Cattle,
pubmed-meshheading:12228313-Cattle Diseases,
pubmed-meshheading:12228313-DNA, Protozoan,
pubmed-meshheading:12228313-Erythrocytes,
pubmed-meshheading:12228313-Genes, Protozoan,
pubmed-meshheading:12228313-Horses,
pubmed-meshheading:12228313-Protein Binding,
pubmed-meshheading:12228313-Protozoan Proteins
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pubmed:year |
2002
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pubmed:articleTitle |
Cellular localization of Babesia bovis merozoite rhoptry-associated protein 1 and its erythrocyte-binding activity.
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pubmed:affiliation |
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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