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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2002-9-12
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pubmed:abstractText |
Bacterial superantigens are extremely potent activators of murine and human T lymphocytes. To engineer superantigens for cancer immunotherapy, staphylococcal enterotoxin A (SEA) was genetically fused to the Fab region of the human colon carcinoma-reactive monoclonal antibody (mAb) C215. Fusion protein C215Fab-SEA can trigger cytotoxic T cells against C215 antigen positive tumor cells and induce tumor-suppressive cytokines. However, the antitumor effect of C215Fab-SEA is often not satisfactory because of T cell deletion after activation and failure to induce potent CTL activity after repeated administration. Lymphotactin (Lptn) is a potent chemoattractant for T cells and NK cells. To improve the therapeutic efficacy of fusion protein C215Fab-SEA we investigated in this study the antitumor responses elicited by combination of C215Fab-SEA and adenovirus-mediated intratumoral Lptn gene transfer in the preestablished C215 antigen expressing B16 melanoma murine model. More significant inhibition of tumor growth and prolonged survival time were observed in tumor-bearing mice that received combined therapy of C215Fab-SEA and Ad-Lptn than those of mice treated with C215Fab-SEA or Ad-Lptn alone. The highest CTL activity of tumor-bearing mice was induced after combined therapy. Intratumoral coadministration of C215Fab-SEA and Ad-Lptn augmented splenic NK activity of tumor-bearing mice most markedly. Our data demonstrate that the in vivo antitumor effect of C215Fab-SEA immunotherapy is potentiated significantly by combination with intratumoral Lptn gene transfer through more efficient induction of specific and nonspecific antitumor immune responses.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, C,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Enterotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Fab Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphokines,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sialoglycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Superantigens,
http://linkedlifedata.com/resource/pubmed/chemical/XCL1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Xcl1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/enterotoxin A, Staphylococcal,
http://linkedlifedata.com/resource/pubmed/chemical/lymphotactin
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0946-2716
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
80
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
585-94
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pubmed:dateRevised |
2011-7-8
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pubmed:meshHeading |
pubmed-meshheading:12226740-Adenoviridae,
pubmed-meshheading:12226740-Animals,
pubmed-meshheading:12226740-Antibodies, Monoclonal,
pubmed-meshheading:12226740-Antigens, Neoplasm,
pubmed-meshheading:12226740-Cell Line,
pubmed-meshheading:12226740-Cell Transplantation,
pubmed-meshheading:12226740-Chemokines, C,
pubmed-meshheading:12226740-Combined Modality Therapy,
pubmed-meshheading:12226740-Cytokines,
pubmed-meshheading:12226740-Enterotoxins,
pubmed-meshheading:12226740-Female,
pubmed-meshheading:12226740-Gene Transfer Techniques,
pubmed-meshheading:12226740-Immunoglobulin Fab Fragments,
pubmed-meshheading:12226740-Killer Cells, Natural,
pubmed-meshheading:12226740-Lymphocyte Activation,
pubmed-meshheading:12226740-Lymphokines,
pubmed-meshheading:12226740-Male,
pubmed-meshheading:12226740-Melanoma, Experimental,
pubmed-meshheading:12226740-Mice,
pubmed-meshheading:12226740-Mice, Inbred C57BL,
pubmed-meshheading:12226740-Neoplasms, Experimental,
pubmed-meshheading:12226740-RNA, Messenger,
pubmed-meshheading:12226740-Recombinant Fusion Proteins,
pubmed-meshheading:12226740-Sialoglycoproteins,
pubmed-meshheading:12226740-Superantigens,
pubmed-meshheading:12226740-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:12226740-Tumor Cells, Cultured
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pubmed:year |
2002
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pubmed:articleTitle |
Adenovirus-mediated intratumoral lymphotactin gene transfer potentiates the antibody-targeted superantigen therapy of cancer.
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pubmed:affiliation |
Institute of Immunology, Zhejiang University, 353 Yan'an Road, Hangzhou 310031, P.R. China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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