| pubmed-article:12221128 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12221128 | lifeskim:mentions | umls-concept:C0672770 | lld:lifeskim |
| pubmed-article:12221128 | lifeskim:mentions | umls-concept:C1705673 | lld:lifeskim |
| pubmed-article:12221128 | lifeskim:mentions | umls-concept:C0205145 | lld:lifeskim |
| pubmed-article:12221128 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:12221128 | lifeskim:mentions | umls-concept:C1166878 | lld:lifeskim |
| pubmed-article:12221128 | lifeskim:mentions | umls-concept:C1332072 | lld:lifeskim |
| pubmed-article:12221128 | lifeskim:mentions | umls-concept:C0915172 | lld:lifeskim |
| pubmed-article:12221128 | lifeskim:mentions | umls-concept:C1156021 | lld:lifeskim |
| pubmed-article:12221128 | lifeskim:mentions | umls-concept:C1624581 | lld:lifeskim |
| pubmed-article:12221128 | lifeskim:mentions | umls-concept:C1999230 | lld:lifeskim |
| pubmed-article:12221128 | lifeskim:mentions | umls-concept:C1293132 | lld:lifeskim |
| pubmed-article:12221128 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:12221128 | pubmed:dateCreated | 2002-9-10 | lld:pubmed |
| pubmed-article:12221128 | pubmed:abstractText | Microtubule assembly is initiated by the gamma-tubulin ring complex (gamma-TuRC). In yeast, the microtubule is nucleated from gamma-TuRC anchored to the amino-terminus of the spindle pole body component Spc110p, which interacts with calmodulin (Cmd1p) at the carboxy-terminus. However, mammalian protein that anchors gamma-TuRC remains to be elucidated. A giant coiled-coil protein, CG-NAP (centrosome and Golgi localized PKN-associated protein), was localized to the centrosome via the carboxyl-terminal region. This region was found to interact with calmodulin by yeast two-hybrid screening, and it shares high homology with the carboxyl-terminal region of another centrosomal coiled-coil protein, kendrin. The amino-terminal region of either CG-NAP or kendrin indirectly associated with gamma-tubulin through binding with gamma-tubulin complex protein 2 (GCP2) and/or GCP3. Furthermore, endogenous CG-NAP and kendrin were coimmunoprecipitated with each other and with endogenous GCP2 and gamma-tubulin, suggesting that CG-NAP and kendrin form complexes and interact with gamma-TuRC in vivo. These proteins were localized to the center of microtubule asters nucleated from isolated centrosomes. Pretreatment of the centrosomes by antibody to CG-NAP or kendrin moderately inhibited the microtubule nucleation; moreover, the combination of these antibodies resulted in stronger inhibition. These results imply that CG-NAP and kendrin provide sites for microtubule nucleation in the mammalian centrosome by anchoring gamma-TuRC. | lld:pubmed |
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| pubmed-article:12221128 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12221128 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12221128 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:12221128 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12221128 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:12221128 | pubmed:issn | 1059-1524 | lld:pubmed |
| pubmed-article:12221128 | pubmed:author | pubmed-author:OnoYoshitakaY | lld:pubmed |
| pubmed-article:12221128 | pubmed:author | pubmed-author:TakahashiMiki... | lld:pubmed |
| pubmed-article:12221128 | pubmed:author | pubmed-author:YamagiwaAkiko... | lld:pubmed |
| pubmed-article:12221128 | pubmed:author | pubmed-author:NishimuraTama... | lld:pubmed |
| pubmed-article:12221128 | pubmed:author | pubmed-author:MukaiHideyuki... | lld:pubmed |
| pubmed-article:12221128 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12221128 | pubmed:volume | 13 | lld:pubmed |
| pubmed-article:12221128 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12221128 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12221128 | pubmed:pagination | 3235-45 | lld:pubmed |
| pubmed-article:12221128 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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