Linked Life Data

12220501

Source:http://linkedlifedata.com/resource/pubmed/id/12220501

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-9-10
pubmed:abstractText
To test the hypothesis that the Kch gene of Escherichia coli encodes a potassium channel, we have transformed E. coli with an expression vector containing the Kch sequence and observed the effect of over-expression of Kch on E. coli. We found that: (i) over-expression of Kch is toxic to E. coli, but the toxicity could be prevented by supplementing the growth medium with K(+), Rb(+), and NH(4)(+), but not Na(+), consistent with the properties of a potassium selective pore; (ii) Cs(+), a blocker of potassium channels, rescues the growth of Kch over-expressing cells; and (iii) when the putative pore-forming region of Kch, containing the signature sequence, was replaced with the corresponding region of the eukaryotic Shaker potassium channel, and the resultant construct expressed in E. coli, the cells became critically dependent on K(+) supply for survival. These data are consistent with the proposed function of Kch, i.e., K(+) conduction.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
13
pubmed:volume
297
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Functional properties of Kch, a prokaryotic homologue of eukaryotic potassium channels.
pubmed:affiliation
School of Biomedical Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't