Source:http://linkedlifedata.com/resource/pubmed/id/12218115
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
2002-9-9
|
| pubmed:abstractText |
Dendritic cells (DC) were found to down-regulate Bcl-2 protein upon maturation in vivo. Because Bcl-2 has been shown to exert anti-apoptotic functions, down-regulation of Bcl-2 could be a mechanism by which DC longevity is controlled. To dysregulate this potential control system and to study the role of Bcl-2 in DC, we expressed human Bcl-2 under control of the murine CD11c-promoter as a transgene specifically in DC and show that DC frequencies and numbers increase in transgenic mice. In vivo bromodeoxyuridin, as well as adoptive, DC transfer studies show that the relative turnover/survival of mature Bcl-2 transgenic DC is increased. This had a direct impact on CD4+ T cell, as well as humoral immune, responses, which were elevated in transgenic animals. When Bcl-2 transgenic DC were used as DC vaccines, they induced 2- to 3-fold greater expansion of Ag-specific CTL, and stronger in vivo cytotoxicity. Overall, these data indicate that down-regulation of Bcl-2 controls DC longevity, which in turn directly regulates immune responses and the efficacy of DC when used as vaccines.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
169
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3006-14
|
| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12218115-Animals,
pubmed-meshheading:12218115-Antibody Formation,
pubmed-meshheading:12218115-Cell Differentiation,
pubmed-meshheading:12218115-Cell Survival,
pubmed-meshheading:12218115-Cells, Cultured,
pubmed-meshheading:12218115-Dendritic Cells,
pubmed-meshheading:12218115-Down-Regulation,
pubmed-meshheading:12218115-Immunity, Cellular,
pubmed-meshheading:12218115-Integrin alphaXbeta2,
pubmed-meshheading:12218115-Lymph Nodes,
pubmed-meshheading:12218115-Lymphocyte Count,
pubmed-meshheading:12218115-Mice,
pubmed-meshheading:12218115-Mice, Inbred C57BL,
pubmed-meshheading:12218115-Mice, Transgenic,
pubmed-meshheading:12218115-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:12218115-Spleen,
pubmed-meshheading:12218115-Thymus Gland,
pubmed-meshheading:12218115-Transgenes,
pubmed-meshheading:12218115-Up-Regulation
|
| pubmed:year |
2002
|
| pubmed:articleTitle |
Bcl-2 controls dendritic cell longevity in vivo.
|
| pubmed:affiliation |
Institute for Immunology, Ludwig-Maximilians University, Munich, Germany.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|