Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2002-9-9
pubmed:abstractText
The branched-chain amino acids (BCAA) are committed to catabolism by the activity of the branched-chain alpha-ketoacid dehydrogenase (BCKD) complex. BCKD activity is regulated through the action of the complex-specific BCKD kinase that phosphorylates two serine residues in the E1alpha subunit. Greater BCKD kinase expression levels result in a lower activity state of BCKD and thus a decreased rate of BCAA catabolism. Activity state varies among tissues and can be altered by diet, exercise, hormones, and disease state. Within individual tissues, the concentration of BCKD kinase reflects the activity state of the BCKD complex. Here we investigated the effects of insulin, an important regulator of hepatic metabolic enzymes, on BCKD kinase expression in Clone 9 rat cells. Insulin effected a twofold increase in message levels and a twofold increase in BCKD kinase protein levels. The response was completely blocked by treatment with LY-294002 and partially blocked by rapamycin, thus demonstrating a dependence on phosphatidylinositol 3-kinase and mTOR function, respectively. These studies suggest that insulin acts to regulate BCAA catabolism through stimulation of BCKD kinase expression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/2-(4-morpholinyl)-8-phenyl-4H-1-benz..., http://linkedlifedata.com/resource/pubmed/chemical/3-Methyl-2-Oxobutanoate..., http://linkedlifedata.com/resource/pubmed/chemical/Blood Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Chromones, http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Hypoglycemic Agents, http://linkedlifedata.com/resource/pubmed/chemical/Insulin, http://linkedlifedata.com/resource/pubmed/chemical/Ketone Oxidoreductases, http://linkedlifedata.com/resource/pubmed/chemical/Morpholines, http://linkedlifedata.com/resource/pubmed/chemical/Multienzyme Complexes, http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0193-1849
pubmed:author
pubmed:issnType
Print
pubmed:volume
283
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
E853-60
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:12217904-3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide), pubmed-meshheading:12217904-Animals, pubmed-meshheading:12217904-Blood Proteins, pubmed-meshheading:12217904-Chromones, pubmed-meshheading:12217904-Clone Cells, pubmed-meshheading:12217904-Enzyme Inhibitors, pubmed-meshheading:12217904-Gene Expression Regulation, Enzymologic, pubmed-meshheading:12217904-Hepatocytes, pubmed-meshheading:12217904-Hypoglycemic Agents, pubmed-meshheading:12217904-Insulin, pubmed-meshheading:12217904-Ketone Oxidoreductases, pubmed-meshheading:12217904-Morpholines, pubmed-meshheading:12217904-Multienzyme Complexes, pubmed-meshheading:12217904-Phosphatidylinositol 3-Kinases, pubmed-meshheading:12217904-Phosphorylation, pubmed-meshheading:12217904-RNA, Messenger, pubmed-meshheading:12217904-Rats
pubmed:year
2002
pubmed:articleTitle
Insulin increases branched-chain alpha-ketoacid dehydrogenase kinase expression in Clone 9 rat cells.
pubmed:affiliation
Graduate Program in Nutrition and Health Sciences, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
pubmed:publicationType
Journal Article