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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2002-9-9
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pubmed:abstractText |
Kidney-specific cadherin (Ksp-cadherin) is a tissue-specific member of the cadherin family that is expressed exclusively in the kidney and developing genitourinary tract. Recent studies have shown that the proximal 250 bp of the Ksp-cadherin gene promoter are sufficient to direct tissue-specific gene expression in vivo and in vitro. The proximal 120 bp of the promoter are evolutionarily conserved between mouse and human and contain a DNase I hypersensitive site that is kidney cell specific. At position -55, the promoter contains a consensus recognition site for hepatocyte nuclear factor-1 (HNF-1). Mutations of the consensus HNF-1 site and downstream GC-boxes inhibit promoter activity in transfected cells. HNF-1alpha and HNF-1beta bind specifically to the -55 site, and both proteins transactivate the promoter directly. Expression of Ksp-cadherin is not altered in the kidneys of HNF-1alpha-deficient mice. However, expression of a gain-of-function HNF-1beta mutant stimulates Ksp-cadherin promoter activity in transfected cells, whereas expression of a dominant-negative mutant inhibits activity. These studies identify Ksp-cadherin as the first kidney-specific promoter that has been shown to be regulated by HNF-1beta. Mutations of HNF-1beta, as occur in humans with inherited renal cysts and diabetes, may cause dysregulated Ksp-cadherin promoter activity.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CDH16 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cadherins,
http://linkedlifedata.com/resource/pubmed/chemical/Cdh16 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonuclease I,
http://linkedlifedata.com/resource/pubmed/chemical/HNF1A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/HNF1B protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 1,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 1-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 1-beta,
http://linkedlifedata.com/resource/pubmed/chemical/Hnf1a protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Hnf1b protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1931-857X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
283
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
F839-51
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pubmed:dateRevised |
2011-4-28
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pubmed:meshHeading |
pubmed-meshheading:12217876-Animals,
pubmed-meshheading:12217876-Base Sequence,
pubmed-meshheading:12217876-Blotting, Northern,
pubmed-meshheading:12217876-Cadherins,
pubmed-meshheading:12217876-Cell Nucleus,
pubmed-meshheading:12217876-Cells, Cultured,
pubmed-meshheading:12217876-Conserved Sequence,
pubmed-meshheading:12217876-DNA Mutational Analysis,
pubmed-meshheading:12217876-DNA-Binding Proteins,
pubmed-meshheading:12217876-Deoxyribonuclease I,
pubmed-meshheading:12217876-Gene Expression Regulation,
pubmed-meshheading:12217876-Genes, Reporter,
pubmed-meshheading:12217876-Hepatocyte Nuclear Factor 1,
pubmed-meshheading:12217876-Hepatocyte Nuclear Factor 1-alpha,
pubmed-meshheading:12217876-Hepatocyte Nuclear Factor 1-beta,
pubmed-meshheading:12217876-Humans,
pubmed-meshheading:12217876-Kidney,
pubmed-meshheading:12217876-Mice,
pubmed-meshheading:12217876-Mice, Knockout,
pubmed-meshheading:12217876-Molecular Sequence Data,
pubmed-meshheading:12217876-Mutation,
pubmed-meshheading:12217876-Nuclear Proteins,
pubmed-meshheading:12217876-Nucleotide Mapping,
pubmed-meshheading:12217876-Plasmids,
pubmed-meshheading:12217876-Promoter Regions, Genetic,
pubmed-meshheading:12217876-Protein Binding,
pubmed-meshheading:12217876-Transcription Factors,
pubmed-meshheading:12217876-Transfection
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pubmed:year |
2002
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pubmed:articleTitle |
Regulation of kidney-specific Ksp-cadherin gene promoter by hepatocyte nuclear factor-1beta.
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pubmed:affiliation |
Division of Nephrology, Department of Internal Medicine, University of Texas Southwestern Medical Center at Dallas, 75390, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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