Linked Life Data

12215426

Source:http://linkedlifedata.com/resource/pubmed/id/12215426

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
45
pubmed:dateCreated
2002-11-4
pubmed:abstractText
The human endometrium is a unique tissue that is periodically shed during menstruation. Although overall triggered by ovarian steroids withdrawal, menstrual induction of matrix metalloproteinases (MMPs) and resulting tissue breakdown are focal responses, pointing to additional local modulators. LEFTY-A, a novel member of the transforming growth factor-beta family identified originally as an endometrial bleeding-associated factor (EBAF), is a candidate for this local control. We measured LEFTY-A and beta-ACTIN mRNA concentration during the menstrual cycle in vivo and found that their ratio was dramatically ( approximately 100-fold) increased at the perimenstrual phase. A similar increase was seen when proliferative explants were cultured for 24 h in the absence of ovarian steroids; this was followed by spontaneous production of proMMP-1, -3, and -9. Both responses were inhibited by progesterone. Moreover, addition of recombinant LEFTY-A to proliferative explants was sufficient to stimulate the expression of proMMP-3 and -7; this response was also blocked by ovarian steroids. Collectively, these data indicate that LEFTY-A may provide a crucial signal for endometrial breakdown and bleeding by triggering expression of several MMPs. Progesterone appears to exert a dual block, upstream by inhibiting LEFTY-A expression and downstream by suppressing its stimulatory effect on MMPs.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
8
pubmed:volume
277
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
42496-504
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:12215426-Actins, pubmed-meshheading:12215426-Base Sequence, pubmed-meshheading:12215426-DNA Primers, pubmed-meshheading:12215426-Endometrium, pubmed-meshheading:12215426-Female, pubmed-meshheading:12215426-Gene Expression Regulation, pubmed-meshheading:12215426-Humans, pubmed-meshheading:12215426-Left-Right Determination Factors, pubmed-meshheading:12215426-Matrix Metalloproteinases, pubmed-meshheading:12215426-Menstrual Cycle, pubmed-meshheading:12215426-Organ Culture Techniques, pubmed-meshheading:12215426-Polymerase Chain Reaction, pubmed-meshheading:12215426-Progesterone, pubmed-meshheading:12215426-RNA, Messenger, pubmed-meshheading:12215426-Recombinant Proteins, pubmed-meshheading:12215426-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12215426-Tissue Inhibitor of Metalloproteinases, pubmed-meshheading:12215426-Transforming Growth Factor beta
pubmed:year
2002
pubmed:articleTitle
Regulation and function of LEFTY-A/EBAF in the human endometrium. mRNA expression during the menstrual cycle, control by progesterone, and effect on matrix metalloprotineases.
pubmed:affiliation
Cell Biology Unit, Christian de Duve Institute of Cellular Pathology, Université catholique de Louvain, Avenue Hippocrate, 75, B-1200 Bruxelles, Belgium.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't