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rdf:type |
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lifeskim:mentions |
umls-concept:C0019704,
umls-concept:C0026809,
umls-concept:C0042210,
umls-concept:C0449432,
umls-concept:C0596988,
umls-concept:C0598434,
umls-concept:C1179435,
umls-concept:C1514562,
umls-concept:C1524073,
umls-concept:C1527148,
umls-concept:C1548799,
umls-concept:C1705248,
umls-concept:C2603343
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pubmed:issue |
27-28
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pubmed:dateCreated |
2002-9-5
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pubmed:abstractText |
In an effort to develop a safe Nef component for use in Cytotoxic T-lymphocyte (CTL)-based HIV-1 vaccines, several versions of Nef constructs lacking myristoylation and dileucine motif were engineered and their abilities to elicit T cell responses were evaluated in mice. Nef-specific murine T cell epitopes were first mapped in three strains of mice (Balb/c, C3H/HeN and C57BL/6), and a pair of dominant Nef-specific CD4(+) and CD8(+) T cell epitopes were identified in C57BL/6 mice. C57BL/6 mice were subsequently immunized with engineered Nef DNA constructs, and Nef-specific CD4(+) and CD8(+) T cell responses were determined. A Nef mutant with simple alanine substitutions at the myristoylation and dileucine sites was impaired in its ability to elicit Nef-specific CD4(+) and CD8(+) T cell responses. Addition of human tissue plasminogen activator (TPA) leader sequence to the N terminus of Nef, which concomitantly inactivates the myristoylation site, significantly enhanced the Nef-specific T cell responses. These findings may have practical implications for developing HIV-1 Nef vaccine component.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AIDS Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Dipeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, nef,
http://linkedlifedata.com/resource/pubmed/chemical/Myristic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Synthetic,
http://linkedlifedata.com/resource/pubmed/chemical/leucylleucine,
http://linkedlifedata.com/resource/pubmed/chemical/nef Gene Products, Human...
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0264-410X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3413-21
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:12213412-AIDS Vaccines,
pubmed-meshheading:12213412-Amino Acid Motifs,
pubmed-meshheading:12213412-Amino Acid Sequence,
pubmed-meshheading:12213412-Amino Acid Substitution,
pubmed-meshheading:12213412-Animals,
pubmed-meshheading:12213412-Base Sequence,
pubmed-meshheading:12213412-CD4-Positive T-Lymphocytes,
pubmed-meshheading:12213412-CD8-Positive T-Lymphocytes,
pubmed-meshheading:12213412-DNA, Viral,
pubmed-meshheading:12213412-Dipeptides,
pubmed-meshheading:12213412-Epitopes,
pubmed-meshheading:12213412-Female,
pubmed-meshheading:12213412-Gene Products, nef,
pubmed-meshheading:12213412-Genes, nef,
pubmed-meshheading:12213412-HIV-1,
pubmed-meshheading:12213412-Humans,
pubmed-meshheading:12213412-Mice,
pubmed-meshheading:12213412-Mice, Inbred C3H,
pubmed-meshheading:12213412-Mice, Inbred C57BL,
pubmed-meshheading:12213412-Molecular Sequence Data,
pubmed-meshheading:12213412-Myristic Acids,
pubmed-meshheading:12213412-Protein Engineering,
pubmed-meshheading:12213412-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:12213412-Vaccines, Synthetic,
pubmed-meshheading:12213412-nef Gene Products, Human Immunodeficiency Virus
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pubmed:year |
2002
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pubmed:articleTitle |
Development of HIV-1 Nef vaccine components: immunogenicity study of Nef mutants lacking myristoylation and dileucine motif in mice.
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pubmed:affiliation |
Merck Research Laboratories, Department of Virus & Cell Biology, Merck and Co. Inc., Sumneytown Pike, P.O. Box 4, West Point, PA 19486, USA. xiaoping_liang@merck.com
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pubmed:publicationType |
Journal Article
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