| pubmed-article:12210080 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12210080 | lifeskim:mentions | umls-concept:C0019168 | lld:lifeskim |
| pubmed-article:12210080 | lifeskim:mentions | umls-concept:C2239176 | lld:lifeskim |
| pubmed-article:12210080 | lifeskim:mentions | umls-concept:C0736268 | lld:lifeskim |
| pubmed-article:12210080 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:12210080 | lifeskim:mentions | umls-concept:C1519522 | lld:lifeskim |
| pubmed-article:12210080 | lifeskim:mentions | umls-concept:C1282914 | lld:lifeskim |
| pubmed-article:12210080 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:12210080 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:12210080 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:12210080 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:12210080 | pubmed:dateCreated | 2002-9-4 | lld:pubmed |
| pubmed-article:12210080 | pubmed:abstractText | Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide and is highly associated with chronic liver disease, including hepatitis B viral infection. In order to study the association between hepatitis B virus (HBV) infection and HCC development, tissue microarrays were used to detect the expression of hepatitis B surface antigen (HBsAg) in 194 HCCs and their surrounding liver tissues, using anti-HBsAg monoclonal antibody. The results showed that the expression of HBsAg is significantly lower in tumour tissue than in non-tumour tissue. Among the 138 cases with positive serum HBsAg, expression of HBsAg was more frequently detected in non-tumour tissue (103 cases, 75%) than in tumour tissue (11 cases, 8%). RT-PCR and Southern blot analysis were performed to explore the mechanism of the decreased expression of HBsAg in tumour cells. The RT-PCR results showed that absence or decreased expression of the HBV S gene was detected in 3/15 (20%) and 6/15 (40%) HCCs, respectively. Integration of HBV in 23 pairs of HCCs and their matched non-tumour liver tissues was studied by Southern blot. The results showed that the integrated HBV S gene sequence was detected in 19/23 tumours (83%) and 1/23 non-tumour tissues (4%), whereas the free replicative virus form was observed in 3/23 tumours (13%) and 14/23 non-tumour tissues (61%). These findings suggest that HBsAg-negative results in tumour tissues were directly related to HBV DNA insertion and provide new insights into the involvement of HBsAg in hepatocarcinogenesis. | lld:pubmed |
| pubmed-article:12210080 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12210080 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12210080 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12210080 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12210080 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12210080 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:12210080 | pubmed:issn | 0022-3417 | lld:pubmed |
| pubmed-article:12210080 | pubmed:author | pubmed-author:TURJ JJJ | lld:pubmed |
| pubmed-article:12210080 | pubmed:author | pubmed-author:WangYiY | lld:pubmed |
| pubmed-article:12210080 | pubmed:author | pubmed-author:ShamJonathan... | lld:pubmed |
| pubmed-article:12210080 | pubmed:author | pubmed-author:FangYanY | lld:pubmed |
| pubmed-article:12210080 | pubmed:author | pubmed-author:GuanXin-YuanX... | lld:pubmed |
| pubmed-article:12210080 | pubmed:author | pubmed-author:WuMeng-ChaoMC | lld:pubmed |
| pubmed-article:12210080 | pubmed:author | pubmed-author:TaiLai-ShanLS | lld:pubmed |
| pubmed-article:12210080 | pubmed:author | pubmed-author:WuWei-QingWQ | lld:pubmed |
| pubmed-article:12210080 | pubmed:copyrightInfo | Copyright 2002 John Wiley & Sons, Ltd. | lld:pubmed |
| pubmed-article:12210080 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12210080 | pubmed:volume | 197 | lld:pubmed |
| pubmed-article:12210080 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12210080 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12210080 | pubmed:pagination | 610-6 | lld:pubmed |
| pubmed-article:12210080 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:12210080 | pubmed:meshHeading | pubmed-meshheading:12210080... | lld:pubmed |
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| pubmed-article:12210080 | pubmed:meshHeading | pubmed-meshheading:12210080... | lld:pubmed |
| pubmed-article:12210080 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12210080 | pubmed:articleTitle | Different expression of hepatitis B surface antigen between hepatocellular carcinoma and its surrounding liver tissue, studied using a tissue microarray. | lld:pubmed |
| pubmed-article:12210080 | pubmed:affiliation | Eastern Hepatobilliary Surgery Hospital, The Second Military Medical University, Shanghai, China. | lld:pubmed |
| pubmed-article:12210080 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12210080 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12210080 | lld:pubmed |
