Source:http://linkedlifedata.com/resource/pubmed/id/12210080
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2002-9-4
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pubmed:abstractText |
Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide and is highly associated with chronic liver disease, including hepatitis B viral infection. In order to study the association between hepatitis B virus (HBV) infection and HCC development, tissue microarrays were used to detect the expression of hepatitis B surface antigen (HBsAg) in 194 HCCs and their surrounding liver tissues, using anti-HBsAg monoclonal antibody. The results showed that the expression of HBsAg is significantly lower in tumour tissue than in non-tumour tissue. Among the 138 cases with positive serum HBsAg, expression of HBsAg was more frequently detected in non-tumour tissue (103 cases, 75%) than in tumour tissue (11 cases, 8%). RT-PCR and Southern blot analysis were performed to explore the mechanism of the decreased expression of HBsAg in tumour cells. The RT-PCR results showed that absence or decreased expression of the HBV S gene was detected in 3/15 (20%) and 6/15 (40%) HCCs, respectively. Integration of HBV in 23 pairs of HCCs and their matched non-tumour liver tissues was studied by Southern blot. The results showed that the integrated HBV S gene sequence was detected in 19/23 tumours (83%) and 1/23 non-tumour tissues (4%), whereas the free replicative virus form was observed in 3/23 tumours (13%) and 14/23 non-tumour tissues (61%). These findings suggest that HBsAg-negative results in tumour tissues were directly related to HBV DNA insertion and provide new insights into the involvement of HBsAg in hepatocarcinogenesis.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-3417
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pubmed:author | |
pubmed:copyrightInfo |
Copyright 2002 John Wiley & Sons, Ltd.
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pubmed:issnType |
Print
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pubmed:volume |
197
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
610-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12210080-Adolescent,
pubmed-meshheading:12210080-Adult,
pubmed-meshheading:12210080-Aged,
pubmed-meshheading:12210080-Blotting, Southern,
pubmed-meshheading:12210080-Carcinoma, Hepatocellular,
pubmed-meshheading:12210080-Female,
pubmed-meshheading:12210080-Hepatitis B, Chronic,
pubmed-meshheading:12210080-Hepatitis B Surface Antigens,
pubmed-meshheading:12210080-Hepatitis B virus,
pubmed-meshheading:12210080-Humans,
pubmed-meshheading:12210080-Liver,
pubmed-meshheading:12210080-Liver Neoplasms,
pubmed-meshheading:12210080-Male,
pubmed-meshheading:12210080-Middle Aged,
pubmed-meshheading:12210080-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:12210080-Virus Integration
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pubmed:year |
2002
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pubmed:articleTitle |
Different expression of hepatitis B surface antigen between hepatocellular carcinoma and its surrounding liver tissue, studied using a tissue microarray.
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pubmed:affiliation |
Eastern Hepatobilliary Surgery Hospital, The Second Military Medical University, Shanghai, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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