Linked Life Data

12209856

Source:http://linkedlifedata.com/resource/pubmed/id/12209856

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-9-4
pubmed:abstractText
Tachykinins depolarize guinea pig intracardiac neurons by activating nonselective cationic channels. Recently, members of the transient receptor potential family of membrane channels (TRPC) have been implicated in the generation of G protein-coupled receptor-activated nonselective cationic currents. We have investigated whether guinea pig cardiac neurons exhibit immunoreactivity to TRPC. Our results showed that nerve fibers within guinea pig intrinsic cardiac ganglia exhibited immunoreactivity to TRPC6. After culture of cardiac ganglia whole-mount explants for 72 hours, the TRPC6-IR fiber networks were absent. Therefore, the TRPC6-IR fibers were derived from sources extrinsic to the heart. A small percentage ( approximately 3%) of intracardiac neurons also exhibited TRPC6 immunoreactivity in control preparations, and the percentage of cells exhibiting TRPC6 immunoreactivity was not changed following explant culture for 72 hours. The few intrinsic TRPC6-IR neurons also exhibited nitric oxide synthase (NOS) immunoreactivity, indicating that they were nitrergic as well. We compared the immunohistochemical staining patterns of TRPC6-IR fibers with the staining patterns of a number of other neurotransmitters or neurotransmitter synthetic enzymes that mark specific extrinsic inputs to the intrinsic cardiac ganglia. The TRPC6-IR fibers were not immunoreactive for choline acetyltransferase, tyrosine hydroxylase, or substance P. However, the TRPC6-IR fibers exhibited immunoreactivity to neuronal NOS. Therefore, we propose that the TRPC6-IR fibers within the guinea pig intrinsic cardiac ganglia are vagal sensory fibers that also contain NOS. We found, in support of this conclusion, that TRPC6-IR cells were also present in sections of nodose ganglia.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9967
pubmed:author
pubmed:copyrightInfo
Copyright 2002 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
26
pubmed:volume
450
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
283-91
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:12209856-Animals, pubmed-meshheading:12209856-Axons, pubmed-meshheading:12209856-Calcium Channels, pubmed-meshheading:12209856-Cells, Cultured, pubmed-meshheading:12209856-Choline O-Acetyltransferase, pubmed-meshheading:12209856-Female, pubmed-meshheading:12209856-Ganglia, Parasympathetic, pubmed-meshheading:12209856-Guinea Pigs, pubmed-meshheading:12209856-Heart, pubmed-meshheading:12209856-Immunohistochemistry, pubmed-meshheading:12209856-Male, pubmed-meshheading:12209856-Myocardium, pubmed-meshheading:12209856-Neurons, Afferent, pubmed-meshheading:12209856-Nitrergic Neurons, pubmed-meshheading:12209856-Nitric Oxide, pubmed-meshheading:12209856-Nitric Oxide Synthase, pubmed-meshheading:12209856-Nodose Ganglion, pubmed-meshheading:12209856-Sympathetic Fibers, Postganglionic, pubmed-meshheading:12209856-Tyrosine 3-Monooxygenase, pubmed-meshheading:12209856-Vagus Nerve, pubmed-meshheading:12209856-Visceral Afferents
pubmed:year
2002
pubmed:articleTitle
TRPC6 immunoreactivity is colocalized with neuronal nitric oxide synthase in extrinsic fibers innervating guinea pig intrinsic cardiac ganglia.
pubmed:affiliation
Department of Anatomy and Neurobiology, University of Vermont College of Medicine, Burlington, Vermont 05405, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.