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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2002-9-3
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pubmed:abstractText |
The effect of nordihydroguaiaretic acid (NDGA) on Ca(2+) signaling in human hepatoma cells (HA22/VGH) has been investigated. NDGA (5-50 microM) increased [Ca(2+)](i) concentration-dependently. The [Ca(2+)](i) increase comprised an initial rise and an elevated phase over a time period of 4 min. Removal of extracellular Ca(2+) reduced 10-50 microM NDGA induced [Ca(2+)](i) signals by 45+/-5%. Consistently, the 50 microM NDGA-induced [Ca(2+)](i) increase in Ca(2+)-containing medium was reduced by 41+/-2% by 10 microM of La(3+), nifedipine or verapamil. In Ca(2+)-free medium, pretreatment with 20 microM NDGA for 6 min abolished the [Ca(2+)](i) increase induced by the endoplasmic reticulum Ca(2+) pump inhibitor thapsigargin (1 microM). Conversely, 20 microM NDGA failed to increase [Ca(2+)](i) after 1 microM thapsigargin had depleted the endoplasmic reticulum Ca(2+) store. Inhibition of phospholipase C with 2 microM U73122 had little effect on 20 microM NDGA-induced Ca(2+) release. Several other lipoxygenase inhibitors had no effect on basal [Ca(2+)](i). Together, the data suggest that NDGA increased [Ca(2+)](i) in hepatocytes in a lipoxygenase-independent manner, by releasing Ca(2+) from the endoplasmic reticulum and causing Ca(2+) influx.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-(6-((3-methoxyestra-1,3,5(10)-trie...,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Estrenes,
http://linkedlifedata.com/resource/pubmed/chemical/Fura-2,
http://linkedlifedata.com/resource/pubmed/chemical/Histamine,
http://linkedlifedata.com/resource/pubmed/chemical/Lanthanum,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoxygenase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Nifedipine,
http://linkedlifedata.com/resource/pubmed/chemical/Nordihydroguaiaretic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrrolidinones,
http://linkedlifedata.com/resource/pubmed/chemical/Thapsigargin,
http://linkedlifedata.com/resource/pubmed/chemical/Verapamil
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0887-2333
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
485-90
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pubmed:dateRevised |
2009-4-10
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pubmed:meshHeading |
pubmed-meshheading:12206814-Calcium,
pubmed-meshheading:12206814-Calcium Signaling,
pubmed-meshheading:12206814-Cell Line,
pubmed-meshheading:12206814-Dose-Response Relationship, Drug,
pubmed-meshheading:12206814-Drug Interactions,
pubmed-meshheading:12206814-Estrenes,
pubmed-meshheading:12206814-Fura-2,
pubmed-meshheading:12206814-Hepatocytes,
pubmed-meshheading:12206814-Histamine,
pubmed-meshheading:12206814-Humans,
pubmed-meshheading:12206814-Lanthanum,
pubmed-meshheading:12206814-Lipoxygenase Inhibitors,
pubmed-meshheading:12206814-Nifedipine,
pubmed-meshheading:12206814-Nordihydroguaiaretic Acid,
pubmed-meshheading:12206814-Pyrrolidinones,
pubmed-meshheading:12206814-Thapsigargin,
pubmed-meshheading:12206814-Verapamil
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pubmed:year |
2002
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pubmed:articleTitle |
Effect of nordihydroguaiaretic acid on intracellular Ca(2+) concentrations in hepatocytes.
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pubmed:affiliation |
Department of Medicine, Kaohsiung Veterans General Hospital, 386 Ta Chung 1st Road, Kaohsiung 813, Taiwan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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