Source:http://linkedlifedata.com/resource/pubmed/id/12205672
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2002-9-2
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| pubmed:abstractText |
The nestin gene is expressed in many CNS stem/progenitor cells, both in the embryo and the adult, and nestin is used commonly as a marker for these cells. In this report we analyze nestin enhancer requirements in the adult CNS, using transgenic mice carrying reporter genes linked to three different nestin enhancer constructs: the genomic rat nestin gene and 5 kb of upstream nestin sequence (NesPlacZ/3), 636 bp of the rat nestin second intron (E/nestin:EGFP), and a corresponding 714 bp region from the human second intron (Nes714tk/lacZ). NesPlacZ/3 and E/nestin:EGFP mice showed reporter gene expression in stem cell-containing regions of brain and spinal cord during normal conditions. NesPlacZ/3 and E/nestin:EGFP mice showed increased expression in spinal cord after injury and NesPlacZ/3 mice displayed elevated expression in the periventricular area of the brain after injury, which was not the case for the E/nestin:EGFP mice. In contrast, no expression in adult CNS in vivo was seen in the Nes714tk/lacZ mice carrying the human enhancer, neither during normal conditions nor after injury. The Nes714 tk/lacZ mice, however, expressed the reporter gene in reactive astrocytes and CNS stem cells cultured ex vivo. Collectively, this suggests a species difference for the nestin enhancer function in adult CNS and that elements outside the second intron enhancer are required for the full injury response in vivo.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
0360-4012
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2002 Wiley-Liss, Inc.
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| pubmed:issnType |
Print
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| pubmed:day |
15
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| pubmed:volume |
69
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
784-94
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:12205672-Age Factors,
pubmed-meshheading:12205672-Animals,
pubmed-meshheading:12205672-Astrocytes,
pubmed-meshheading:12205672-Brain Injuries,
pubmed-meshheading:12205672-Enhancer Elements, Genetic,
pubmed-meshheading:12205672-Epidermal Growth Factor,
pubmed-meshheading:12205672-Female,
pubmed-meshheading:12205672-Gene Expression,
pubmed-meshheading:12205672-Genes, Reporter,
pubmed-meshheading:12205672-Humans,
pubmed-meshheading:12205672-Intermediate Filament Proteins,
pubmed-meshheading:12205672-Introns,
pubmed-meshheading:12205672-Lac Operon,
pubmed-meshheading:12205672-Male,
pubmed-meshheading:12205672-Mice,
pubmed-meshheading:12205672-Mice, Inbred C57BL,
pubmed-meshheading:12205672-Mice, Inbred CBA,
pubmed-meshheading:12205672-Mice, Transgenic,
pubmed-meshheading:12205672-Nerve Tissue Proteins,
pubmed-meshheading:12205672-Neurons,
pubmed-meshheading:12205672-Spinal Cord Injuries,
pubmed-meshheading:12205672-Stem Cells,
pubmed-meshheading:12205672-Tumor Cells, Cultured
|
| pubmed:year |
2002
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| pubmed:articleTitle |
Nestin enhancer requirements for expression in normal and injured adult CNS.
|
| pubmed:affiliation |
Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institute, Stockholm, Sweden.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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