Linked Life Data

12204414

Source:http://linkedlifedata.com/resource/pubmed/id/12204414

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2002-9-2
pubmed:abstractText
This paper describes the effective determination of DNA scission site using a novel approach that is based on the Sanger method of nucleotide sequencing. The DNA scission site is determined by contrast with the nucleotide sequence of the DNA. Here, instead of the traditional Maxam-Gilbert method for the determination of the DNA sequence, we utilized the Sanger method and studied its effectiveness in the determination of DNA scission sites. Using this method, the determination of DNA scission site becomes more facile and exact. And the total time for the determination is reduced nearly by half in comparison to the Maxam-Gilbert method. Further advantages of this novel approach include the reduced risks of radiation exposure for researchers and contamination of the apparatus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0165-022X
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
52
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
97-109
pubmed:dateRevised
2006-5-1
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Novel approach for the effective determination of DNA scission site using the Sanger method.
pubmed:affiliation
Graduate School of Science and Engineering, Ibaraki University, Mito, Ibaraki 310-8512, Japan.
pubmed:publicationType
Journal Article