Linked Life Data

12202562

Source:http://linkedlifedata.com/resource/pubmed/id/12202562

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2002-8-30
pubmed:abstractText
A real-time reverse transcriptase PCR (RRT-PCR) assay based on the avian influenza virus matrix gene was developed for the rapid detection of type A influenza virus. Additionally, H5 and H7 hemagglutinin subtype-specific probe sets were developed based on North American avian influenza virus sequences. The RRT-PCR assay utilizes a one-step RT-PCR protocol and fluorogenic hydrolysis type probes. The matrix gene RRT-PCR assay has a detection limit of 10 fg or approximately 1,000 copies of target RNA and can detect 0.1 50% egg infective dose of virus. The H5- and H7-specific probe sets each have a detection limit of 100 fg of target RNA or approximately 10(3) to 10(4) gene copies. The sensitivity and specificity of the real-time PCR assay were directly compared with those of the current standard for detection of influenza virus: virus isolation (VI) in embryonated chicken eggs and hemagglutinin subtyping by hemagglutination inhibition (HI) assay. The comparison was performed with 1,550 tracheal and cloacal swabs from various avian species and environmental swabs obtained from live-bird markets in New York and New Jersey. Influenza virus-specific RRT-PCR results correlated with VI results for 89% of the samples. The remaining samples were positive with only one detection method. Overall the sensitivity and specificity of the H7- and H5-specific RRT-PCR were similar to those of VI and HI.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12202562-10196246, http://linkedlifedata.com/resource/pubmed/commentcorrection/12202562-10861198, http://linkedlifedata.com/resource/pubmed/commentcorrection/12202562-10921836, http://linkedlifedata.com/resource/pubmed/commentcorrection/12202562-11136770, http://linkedlifedata.com/resource/pubmed/commentcorrection/12202562-11266220, http://linkedlifedata.com/resource/pubmed/commentcorrection/12202562-11332492, http://linkedlifedata.com/resource/pubmed/commentcorrection/12202562-11448033, http://linkedlifedata.com/resource/pubmed/commentcorrection/12202562-11483213, http://linkedlifedata.com/resource/pubmed/commentcorrection/12202562-1871133, http://linkedlifedata.com/resource/pubmed/commentcorrection/12202562-4004223, http://linkedlifedata.com/resource/pubmed/commentcorrection/12202562-4796550, http://linkedlifedata.com/resource/pubmed/commentcorrection/12202562-6516214, http://linkedlifedata.com/resource/pubmed/commentcorrection/12202562-7483266, http://linkedlifedata.com/resource/pubmed/commentcorrection/12202562-8757992, http://linkedlifedata.com/resource/pubmed/commentcorrection/12202562-9660072, http://linkedlifedata.com/resource/pubmed/commentcorrection/12202562-9889212
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3256-60
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Development of a real-time reverse transcriptase PCR assay for type A influenza virus and the avian H5 and H7 hemagglutinin subtypes.
pubmed:affiliation
Southeast Poultry Research Laboratory, USDA Agricultural Research Service, Athens, Georgia 30605, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Evaluation Studies