Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2002-8-30
pubmed:abstractText
The cytolethal distending toxin (Cdt) of Actinobacillus actinomycetemcomitans, a periodontal pathogen, is a newly described cytotoxin with immunosuppressive properties, capable of causing cell cycle arrest of lymphocytes. The objectives of this study were to investigate the occurrence of A. actinomycetemcomitans with the cdt genotype in the subgingival plaque of periodontitis patients and to determine the association of this bacterial genotype with periodontal disease. A total of 146 subgingival plaque samples from periodontitis patients were assayed by the PCR method using oligonucleotide primers targeting the cdt operon of A. actinomycetemcomitans. Primers targeting the leukotoxin gene A (ltxA) of A. actinomycetemcomitans was used to determine the occurrence of the bacteria in the plaque samples at baseline. At baseline, A. actinomycetemcomitans was detected in 106 out of 146 (73%) diseased sites studied. Among the 106 diseased sites found to harbor A. actinomycetemcomitans, 13 sites were positive for the bacteria with the cdt genotype (12%). Out of the 13 positive sites, 10 sites were obtained from patients diagnosed with aggressive periodontitis (77%). Thus, A. actinomycetemcomitans with the cdt genetic subtype has low occurrence in the subgingival plaque of periodontitis patients. However, a strong association was observed between the presence of the bacteria and aggressive forms of periodontitis. Thus, the cytotoxic and immunosuppressive properties of A. actinomycetemcomitans Cdt may function to cripple the host immunity and contribute to the pathogenesis of aggressive periodontitis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
D
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0022-3484
pubmed:author
pubmed:issnType
Print
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
268-72
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12200970-Actinobacillus actinomycetemcomitans, pubmed-meshheading:12200970-Adult, pubmed-meshheading:12200970-Bacterial Proteins, pubmed-meshheading:12200970-Bacterial Toxins, pubmed-meshheading:12200970-DNA Primers, pubmed-meshheading:12200970-Dental Plaque, pubmed-meshheading:12200970-Exotoxins, pubmed-meshheading:12200970-Genotype, pubmed-meshheading:12200970-Hemolysin Proteins, pubmed-meshheading:12200970-Humans, pubmed-meshheading:12200970-Immunosuppressive Agents, pubmed-meshheading:12200970-Lymphocytes, pubmed-meshheading:12200970-Middle Aged, pubmed-meshheading:12200970-Operon, pubmed-meshheading:12200970-Periodontal Diseases, pubmed-meshheading:12200970-Polymerase Chain Reaction, pubmed-meshheading:12200970-Polymorphism, Restriction Fragment Length, pubmed-meshheading:12200970-Protein Subunits, pubmed-meshheading:12200970-Substrate Specificity
pubmed:year
2002
pubmed:articleTitle
Cytolethal distending toxin of Actinobacillus actinomycetemcomitans. Occurrence and association with periodontal disease.
pubmed:affiliation
Department of Microbiology, Faculty of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117597, Republic of Singapore.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't