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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2002-8-29
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pubmed:abstractText |
Mutagenesis by homologous recombination was evaluated in Xylella fastidiosa by using the bga gene, coding for beta-galactosidase, as a model. Integration of replicative plasmids by homologous recombination between the cloned truncated copy of bga and the endogenous gene was produced by one or two crossover events leading to beta-galactosidase mutants. A promoterless chloramphenicol acetyltransferase gene was used to monitor the expression of the target gene and to select a cvaB mutant.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12200328-10910347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12200328-11050442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12200328-11119303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12200328-11319110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12200328-11386365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12200328-11466274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12200328-15012538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12200328-1779931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12200328-1937792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12200328-8352747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12200328-8655485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12200328-8687420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12200328-9172327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12200328-9847197
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0099-2240
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4658-65
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pubmed:dateRevised |
2011-1-2
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pubmed:meshHeading |
pubmed-meshheading:12200328-Alleles,
pubmed-meshheading:12200328-Chloramphenicol,
pubmed-meshheading:12200328-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:12200328-Gammaproteobacteria,
pubmed-meshheading:12200328-Genes, Reporter,
pubmed-meshheading:12200328-Mutagenesis, Insertional,
pubmed-meshheading:12200328-Plasmids,
pubmed-meshheading:12200328-Recombination, Genetic,
pubmed-meshheading:12200328-Replication Origin,
pubmed-meshheading:12200328-beta-Galactosidase
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pubmed:year |
2002
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pubmed:articleTitle |
Gene disruption by homologous recombination in the Xylella fastidiosa citrus variegated chlorosis strain.
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pubmed:affiliation |
Fundo de Defesa da Citricultura (Fundecitrus), Araraquara, São Paulo, Brazil.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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