Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2002-8-29
pubmed:abstractText
Previous experimental studies have shown that high dietary fat intake is associated with mammary carcinogenesis. In the current study, the effect of 5-LOX or 12-LOX inhibitors on human breast cancer cell proliferation and apoptosis, as well as the possible mechanisms were investigated. The LOX inhibitors, NDGA, Rev-5901, and baicalein all inhibited proliferation and induced apoptosis in MCF-7 (ER+) and MDA-MB-231 (ER-) breast cancer cell in vitro. In contrast, the LOX products, 5-HETE and 12-HETE had mitogenic effects, stimulating the proliferation of both cell lines. These inhibitors also induced cytochrome c release, caspase-9 activation, as well as downstream caspase-3, caspase-7 activation, and PARP cleavage. LOX inhibitor treatment also reduced the levels of anti-apoptotic proteins Bcl-2 and Mcl-1 and increased the levels of the pro-apoptotic protein bax. In conclusion, blockade of both 5-LOX and 12-LOX pathways induces apoptosis in breast cancer cells through the cytochrome c release and caspase-9 activation, with changes in the levels of Bcl-2 family proteins.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/12-Hydroxy-5,8,10,14-eicosatetraenoi..., http://linkedlifedata.com/resource/pubmed/chemical/5-hydroxy-6,8,11,14-eicosatetraenoic..., http://linkedlifedata.com/resource/pubmed/chemical/CASP3 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/CASP7 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/CASP9 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3, http://linkedlifedata.com/resource/pubmed/chemical/Caspase 7, http://linkedlifedata.com/resource/pubmed/chemical/Caspase 9, http://linkedlifedata.com/resource/pubmed/chemical/Caspases, http://linkedlifedata.com/resource/pubmed/chemical/Chemotactic Factors, http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome c Group, http://linkedlifedata.com/resource/pubmed/chemical/DNA, http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Hydroxyeicosatetraenoic Acids, http://linkedlifedata.com/resource/pubmed/chemical/Lipoxygenase Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Mitogens, http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Poly(ADP-ribose) Polymerases, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2, http://linkedlifedata.com/resource/pubmed/chemical/myeloid cell leukemia sequence 1...
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
296
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
942-8
pubmed:dateRevised
2008-7-9
pubmed:meshHeading
pubmed-meshheading:12200139-12-Hydroxy-5,8,10,14-eicosatetraenoic Acid, pubmed-meshheading:12200139-Apoptosis, pubmed-meshheading:12200139-Blotting, Western, pubmed-meshheading:12200139-Breast Neoplasms, pubmed-meshheading:12200139-Caspase 3, pubmed-meshheading:12200139-Caspase 7, pubmed-meshheading:12200139-Caspase 9, pubmed-meshheading:12200139-Caspases, pubmed-meshheading:12200139-Cell Division, pubmed-meshheading:12200139-Chemotactic Factors, pubmed-meshheading:12200139-Cytochrome c Group, pubmed-meshheading:12200139-Cytosol, pubmed-meshheading:12200139-DNA, pubmed-meshheading:12200139-Dose-Response Relationship, Drug, pubmed-meshheading:12200139-Enzyme Activation, pubmed-meshheading:12200139-Enzyme Inhibitors, pubmed-meshheading:12200139-Flow Cytometry, pubmed-meshheading:12200139-Humans, pubmed-meshheading:12200139-Hydroxyeicosatetraenoic Acids, pubmed-meshheading:12200139-Lipoxygenase Inhibitors, pubmed-meshheading:12200139-Mitogens, pubmed-meshheading:12200139-Neoplasm Proteins, pubmed-meshheading:12200139-Poly(ADP-ribose) Polymerases, pubmed-meshheading:12200139-Proto-Oncogene Proteins c-bcl-2, pubmed-meshheading:12200139-Tumor Cells, Cultured
pubmed:year
2002
pubmed:articleTitle
The mechanisms of lipoxygenase inhibitor-induced apoptosis in human breast cancer cells.
pubmed:affiliation
Department of Surgery, Gastrointestinal Oncology Laboratories, Northwestern University Medical School, Chicago, IL 60611, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't