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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2002-8-29
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pubmed:abstractText |
A candidate protein for the basolateral peptide transporter of rat jejunum is described. Vascular perfusion of the photoaffinity label, [4-azido-D-phe]-L-ala (2.5mM), had no effect on the transepithelial transport of the non-hydrolysable dipeptide D-phe-L-gln (1mM) from the lumen, its mucosal accumulation or wash-out into the vascular perfusate. When the label was perfused luminally, the transepithelial transport of D-phe-L-gln was inhibited by 38% (P<0.001) and accumulation increased by 62% (P<0.05). These data are consistent with those of a basolateral transporter that is strongly asymmetric in its substrate binding and transport properties. Labelling of basolateral membrane vesicles with [4-azido-3,5-3H-D-phe]-L-ala revealed that the majority of label was incorporated into a single protein of M(r)112+/-2 kDa and pI 6.5. MALDI-TOF analysis of tryptic digests of the protein followed by database searches established that this protein was novel with no obvious similarity to PepT1, the apical membrane transporter.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
296
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
918-22
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:12200135-Animals,
pubmed-meshheading:12200135-Azides,
pubmed-meshheading:12200135-Biological Transport,
pubmed-meshheading:12200135-Carrier Proteins,
pubmed-meshheading:12200135-Cell Membrane,
pubmed-meshheading:12200135-Databases as Topic,
pubmed-meshheading:12200135-Epithelial Cells,
pubmed-meshheading:12200135-Expressed Sequence Tags,
pubmed-meshheading:12200135-Jejunum,
pubmed-meshheading:12200135-Light,
pubmed-meshheading:12200135-Male,
pubmed-meshheading:12200135-Mass Spectrometry,
pubmed-meshheading:12200135-Models, Chemical,
pubmed-meshheading:12200135-Perfusion,
pubmed-meshheading:12200135-Phenylalanine,
pubmed-meshheading:12200135-Photoaffinity Labels,
pubmed-meshheading:12200135-Rats,
pubmed-meshheading:12200135-Rats, Wistar,
pubmed-meshheading:12200135-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:12200135-Symporters,
pubmed-meshheading:12200135-Time Factors,
pubmed-meshheading:12200135-Trypsin
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pubmed:year |
2002
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pubmed:articleTitle |
Identification of a candidate membrane protein for the basolateral peptide transporter of rat small intestine.
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pubmed:affiliation |
Department of Biology, University of York, P.O. Box 373, York YO10 5YW, UK. ejs9@york.ac.uk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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