Linked Life Data

12198354

Source:http://linkedlifedata.com/resource/pubmed/id/12198354

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-8-28
pubmed:abstractText
The authors investigated the expression of basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) during skin wound healing using immunohistochemical techniques. After a full-thickness incision was made on the dorsal skin, mice were killed 0.5, 1, 3, 8, 24, 72, 144, or 240 hours after incision, and the wound was excised. To evaluate the influences of postmortem degeneration, cutaneous wound excision was performed 1 to 5 days after the mice were killed. The excised wounds were stained by the conventional streptoavidin-biotinylated peroxidase complex method, using specific antibodies, and the ratio of the number of positive cells to total cells was determined. Expression of bFGF was detected in the nuclei of epidermal cells and fibroblasts in the early 0.5- to 1-hour phases and the late 24- to 144-hour phases. Expression of VEGF was detected in the cytoplasm of epidermal cells in the 24- to 144-hour phases. Immunoreactivity of both cytokines was detected 1 day post mortem and was especially well preserved in the fibroblasts. Time-dependent expression of both factors suggested that they would be useful markers for the determination of wound age. However, bFGF should be superior to VEGF because of its earlier expression and because of its more persistent expression in dermal fibroblasts with increasing postmortem interval.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0195-7910
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
264-7
pubmed:dateRevised
2011-2-2
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Immunohistochemical study of basic fibroblast growth factor and vascular endothelial growth factor expression for age determination of cutaneous wounds.
pubmed:affiliation
Department of Legal Medicine, Iwate Medical University School of Medicine, Morioka, Japan. mtakamiy@iwate-med.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't