Source:http://linkedlifedata.com/resource/pubmed/id/12196708
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2002-8-27
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| pubmed:abstractText |
We report here the results of fetal cell enrichment from maternal blood in 58 pregnant women by the use of magnetic activated cell sorting (MACS) with erythroblast-specific and/or maternal cell specific antibodies. Two approaches were compared; one-step MACS to enrich CD71+ (a membrane-bound marker) or GPA+ (another marker, glycophorin A) fetal cells versus two-step MACS to deplete CD14+ maternal cells and subsequently to enrich fetal (CD71+ or GPA+) cells. The existence of fetal cells was ensured by both FISH with Y-specific probes and karyotyping of respective amniotic and/or chorionic vullus cells, the results being applied for comparison of detection rate for XY fetuses between the two MACS procedures. In 24 (38.8%) of the 58 blood samples examined, Y-positive cells were observed by FISH, whereas there were 38 true XY fetuses later confirmed by karyotyping, including two cases of 47,XY,+21. On the other hand, in Y-negative cells by FISH, there were two cases of 47,XX,+18. The average number of cells sorted did not differ among one-step MACS procedures with anti-CD14, anti-CD71 and anti-GPA antibodies. With the latter, 12 (75%) of 16 Y-positive fetuses were detected, while only one (20%) of 5 Y-positive fetuses was detected by two-step MACS with anti-CD14/anti-GPA antibodies. The detection rate significantly varied (p = 0.0024) between the two procedures, although the numbers of cases examined were small. There was no statistical difference (p > 0.05) between one-step and two-step MACS with other combinations of antibodies. These findings indicate that one-step MACS using the anti-GPA antibody is more effective than two step MACS for enrichment of fetal cells from maternal blood.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Jun
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| pubmed:issn |
0914-3505
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
42
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
120-4
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12196708-Adult,
pubmed-meshheading:12196708-Antibody Specificity,
pubmed-meshheading:12196708-Antigens, CD,
pubmed-meshheading:12196708-Antigens, Differentiation, B-Lymphocyte,
pubmed-meshheading:12196708-Chromosome Mapping,
pubmed-meshheading:12196708-Chromosomes, Human, Pair 18,
pubmed-meshheading:12196708-Female,
pubmed-meshheading:12196708-Fetal Blood,
pubmed-meshheading:12196708-Fetus,
pubmed-meshheading:12196708-Hematopoietic Stem Cells,
pubmed-meshheading:12196708-Humans,
pubmed-meshheading:12196708-Immunomagnetic Separation,
pubmed-meshheading:12196708-In Situ Hybridization, Fluorescence,
pubmed-meshheading:12196708-Karyotyping,
pubmed-meshheading:12196708-Maternal Age,
pubmed-meshheading:12196708-Pregnancy,
pubmed-meshheading:12196708-Pregnancy, High-Risk,
pubmed-meshheading:12196708-Receptors, Transferrin
|
| pubmed:year |
2002
|
| pubmed:articleTitle |
Enrichment of fetal cells from maternal blood by magnetic activated cell sorting (MACS) with fetal cell specific antibodies: one-step versus two-step MACS.
|
| pubmed:affiliation |
Department of Obstetrics and Gynecology, Nagoya City University Medical School, Aichi 467-8601, Japan. zhaoxx@med.nagoya-cu.ac.jp
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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