Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2002-9-5
pubmed:abstractText
The Saccharomyces cerevisiae mitotic exit network (MEN) is a conserved signaling network that coordinates events associated with the M to G1 transition. We investigated the function of two S. cerevisiae proteins related to the MEN proteins Mob1p and Dbf2p kinase. Previous work indicates that cells lacking the Dbf2p-related protein Cbk1p fail to sustain polarized growth during early bud morphogenesis and mating projection formation (Bidlingmaier, S., E.L. Weiss, C. Seidel, D.G. Drubin, and M. Snyder. 2001. Mol. Cell. Biol. 21:2449-2462). Cbk1p is also required for Ace2p-dependent transcription of genes involved in mother/daughter separation after cytokinesis. Here we show that the Mob1p-related protein Mob2p physically associates with Cbk1p kinase throughout the cell cycle and is required for full Cbk1p kinase activity, which is periodically activated during polarized growth and mitosis. Both Mob2p and Cbk1p localize interdependently to the bud cortex during polarized growth and to the bud neck and daughter cell nucleus during late mitosis. We found that Ace2p is restricted to daughter cell nuclei via a novel mechanism requiring Mob2p, Cbk1p, and a functional nuclear export pathway. Furthermore, nuclear localization of Mob2p and Ace2p does not occur in mob1-77 or cdc14-1 mutants, which are defective in MEN signaling, even when cell cycle arrest is bypassed. Collectively, these data indicate that Mob2p-Cbk1p functions to (a) maintain polarized cell growth, (b) prevent the nuclear export of Ace2p from the daughter cell nucleus after mitotic exit, and (c) coordinate Ace2p-dependent transcription with MEN activation. These findings may implicate related proteins in linking the regulation of cell morphology and cell cycle transitions with cell fate determination and development.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-10074450, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-10219244, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-10517323, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-10970846, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-11014197, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-11025657, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-11027275, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-11166217, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-11257003, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-11259593, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-11262236, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-11309124, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-11378390, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-11404483, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-11564880, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-11747810, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-11832211, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-1652372, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-1730413, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-2005820, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-3302672, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-4116719, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-7588053, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-7768349, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-8602515, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-8625408, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-8625409, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-8978675, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-9323132, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-942051, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-9436989, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-9528782, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-9700152, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-9717241, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-9732290, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-9843569, http://linkedlifedata.com/resource/pubmed/commentcorrection/12196508-9885559
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/ACE2 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/CBK1 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins, http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Histones, http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides..., http://linkedlifedata.com/resource/pubmed/chemical/MOB2 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances, http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:day
2
pubmed:volume
158
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
885-900
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
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