Source:http://linkedlifedata.com/resource/pubmed/id/12195374
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2002-8-26
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| pubmed:abstractText |
The T cell receptor (TCR) repertoires of 24 human immunodeficiency virus (HIV) type 1-infected children were determined by flow cytometry in combination with sequencing of the highly variable TCR complementarity-determining region 3, permitting a quantitative and qualitative assessment of TCR repertoire. Expanded subsets of CD8(+) cells expressing a particular TCR beta-chain variable region were more commonly identified in HIV-1-infected children than in healthy control subjects (75% vs. 13.5%; P<.0001). Older age and lower percentage of CD4(+) cells were correlated with expansions. Oligoclonal populations occupied 71%-95% of each expanded subset, and predominant clones had high absolute counts. There was evidence of functional differentiation to CD28(-) effector cytotoxic T lymphocytes, and cells bearing identical TCRs were identified in both CD28(+) and CD28(-) cell populations. HIV-1 specificity was observed for expanded clones. Children with expansions were not more likely to have increased numbers of CD8(+) T cells, a finding consistent with the possibility that the CD8(+) TCR repertoire has limited diversity.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0022-1899
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
186
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
477-85
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:12195374-Adolescent,
pubmed-meshheading:12195374-Amino Acid Sequence,
pubmed-meshheading:12195374-CD8-Positive T-Lymphocytes,
pubmed-meshheading:12195374-Child,
pubmed-meshheading:12195374-Child, Preschool,
pubmed-meshheading:12195374-Clone Cells,
pubmed-meshheading:12195374-Complementarity Determining Regions,
pubmed-meshheading:12195374-Cytotoxicity, Immunologic,
pubmed-meshheading:12195374-Female,
pubmed-meshheading:12195374-Flow Cytometry,
pubmed-meshheading:12195374-Genes, T-Cell Receptor beta,
pubmed-meshheading:12195374-HIV Infections,
pubmed-meshheading:12195374-HIV-1,
pubmed-meshheading:12195374-Humans,
pubmed-meshheading:12195374-Infant,
pubmed-meshheading:12195374-Leukocytes, Mononuclear,
pubmed-meshheading:12195374-Lymphocyte Activation,
pubmed-meshheading:12195374-Male,
pubmed-meshheading:12195374-Molecular Sequence Data,
pubmed-meshheading:12195374-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:12195374-Sequence Analysis, DNA,
pubmed-meshheading:12195374-T-Lymphocyte Subsets
|
| pubmed:year |
2002
|
| pubmed:articleTitle |
Clonal CD8+ T cell expansions in peripheral blood from human immunodeficiency virus type 1-infected children.
|
| pubmed:affiliation |
Division of Pediatric Infectious Diseases, University of Colorado Health Sciences Center, Denver 80262, USA. betsy.mcfarland@uchsc.edu
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|