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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2002-8-23
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pubmed:abstractText |
IL-10 regulates inflammation by reducing cytokine and chemokine production from activated macrophages. We performed microarray experiments to identify possible effector molecules of IL-10 and to investigate the global effect of IL-10 on the transcriptional response induced in LPS-activated macrophages. To exclude background effects of endogenous IL-10, macrophages from IL-10-deficient mice were used. IL-10 up-regulated expression of a small number of genes (26 and 37 after 45 min and 3 h, respectively), including newly identified and previously documented targets such as suppressor of cytokine signaling-3 and IL-1 receptor antagonist. However, the activation program triggered by LPS was profoundly affected by IL-10. IL-10 repressed 62 and further increased 15 of 259 LPS-induced genes. For all genes examined, the effects of IL-10 were determined to be STAT3-dependent. These results suggest that IL-10 regulates STAT3-dependent pathways that selectively target a broad component of LPS-induced genes at the mRNA level.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arginase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/STAT3 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Stat3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
169
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2253-63
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:12193690-Animals,
pubmed-meshheading:12193690-Arginase,
pubmed-meshheading:12193690-Bone Marrow Cells,
pubmed-meshheading:12193690-DNA-Binding Proteins,
pubmed-meshheading:12193690-Down-Regulation,
pubmed-meshheading:12193690-Gene Expression Regulation,
pubmed-meshheading:12193690-Interleukin-10,
pubmed-meshheading:12193690-Interleukin-4,
pubmed-meshheading:12193690-Interphase,
pubmed-meshheading:12193690-Lipopolysaccharides,
pubmed-meshheading:12193690-Macrophage Activation,
pubmed-meshheading:12193690-Macrophages,
pubmed-meshheading:12193690-Mice,
pubmed-meshheading:12193690-Mice, Inbred C57BL,
pubmed-meshheading:12193690-Mice, Knockout,
pubmed-meshheading:12193690-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:12193690-Receptors, Interleukin-4,
pubmed-meshheading:12193690-STAT3 Transcription Factor,
pubmed-meshheading:12193690-Signal Transduction,
pubmed-meshheading:12193690-Trans-Activators,
pubmed-meshheading:12193690-Up-Regulation
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pubmed:year |
2002
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pubmed:articleTitle |
Shaping gene expression in activated and resting primary macrophages by IL-10.
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pubmed:affiliation |
Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN 38015, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Validation Studies
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