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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
2002-8-23
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pubmed:databankReference |
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pubmed:abstractText |
The small multicopy plasmid pAMalpha1 (9.75 kb) encoding tetracycline resistance in Enterococcus faecalis is known to generate tandem repeats of a 4.1-kb segment carrying tet(L) when cells are grown extensively in the presence of tetracycline. Here we show that the initial (rate-limiting) step involves a site-specific recombination event involving plasmid-encoded relaxase activity acting at two recombination sequences (RS1 and RS2) that flank the tet determinant. We also present the complete nucleotide sequence of pAMalpha1.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-10974113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-10977892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-11251841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-122512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-1657880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-1697573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-1946749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-2156704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-232126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-2345147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-2644236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-2768188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-2842316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-2987186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-3031012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-3132094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-3584064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-401496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-415051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-4202995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-4204433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-6100903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-6268611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-6292164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-6307973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-6311796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-6782074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-6782083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-7005900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-7019717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-7079389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-7574478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-7597101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-807914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-810482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-818395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-8195075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-8515230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-9102462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12193637-9180693
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
184
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5187-93
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12193637-Anti-Bacterial Agents,
pubmed-meshheading:12193637-Bacterial Proteins,
pubmed-meshheading:12193637-Base Sequence,
pubmed-meshheading:12193637-Conjugation, Genetic,
pubmed-meshheading:12193637-Enterococcus faecalis,
pubmed-meshheading:12193637-Gene Amplification,
pubmed-meshheading:12193637-Gene Deletion,
pubmed-meshheading:12193637-Molecular Sequence Data,
pubmed-meshheading:12193637-Mutagenesis, Site-Directed,
pubmed-meshheading:12193637-Plasmids,
pubmed-meshheading:12193637-Recombination, Genetic,
pubmed-meshheading:12193637-Sequence Analysis, DNA,
pubmed-meshheading:12193637-Tetracycline,
pubmed-meshheading:12193637-Tetracycline Resistance,
pubmed-meshheading:12193637-Trans-Activators
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pubmed:year |
2002
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pubmed:articleTitle |
Amplification of the tetracycline resistance determinant of pAMalpha1 in Enterococcus faecalis requires a site-specific recombination event involving relaxase.
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pubmed:affiliation |
Department of Biologic and Materials Sciences, School of Dentistry, The University of Michigan, Ann Arbor, Michigan 48109, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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