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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2002-8-23
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pubmed:abstractText |
We are investigating the mechanisms by which aromatic hydrocarbons, such as 3-methylcholanthrene (MC), suppress hepatic cytochrome P450 2C11 (CYP2C11) gene expression. CYP2C11 is an enzyme expressed in the liver of male rats and is regulated by a pulsatile pattern of GH secretion. We have previously shown that MC attenuates the stimulatory effect of GH on CYP2C11 expression in hypophysectomized male rats. In follow-up studies we evaluated the effect of MC on GH-stimulated signal transducer and activator of transcription 5b (STAT5b) phosphorylation, nuclear translocation, and DNA-binding activity. GH-stimulated increases in hepatic nuclear STAT5b and phospho-STAT5b levels were not different between groups of hypophysectomized rats receiving MC or vehicle. This observation was corroborated at the DNA-binding level by EMSA. We also measured GH-induced STAT5b activation in the H4IIE rat hepatoma cell line. STAT5b DNA-binding activity detected in GH-treated cells was not affected by MC. Immunocytochemistry experiments revealed no effect of MC on GH-stimulated STAT5b nuclear translocation in H4IIE cells. These in vivo and in vitro data suggest that interference with GH-stimulated STAT5b activation does not constitute a mechanism by which MC attenuates the stimulatory effect of GH on CYP2C11 gene expression.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Caseins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Methylcholanthrene,
http://linkedlifedata.com/resource/pubmed/chemical/Milk Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/STAT5 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Stat5b protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0013-7227
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
143
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3284-94
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12193540-Animals,
pubmed-meshheading:12193540-Binding Sites,
pubmed-meshheading:12193540-Biological Transport,
pubmed-meshheading:12193540-Caseins,
pubmed-meshheading:12193540-Cell Nucleus,
pubmed-meshheading:12193540-DNA,
pubmed-meshheading:12193540-DNA-Binding Proteins,
pubmed-meshheading:12193540-Growth Hormone,
pubmed-meshheading:12193540-Hypophysectomy,
pubmed-meshheading:12193540-Immunoblotting,
pubmed-meshheading:12193540-Immunohistochemistry,
pubmed-meshheading:12193540-Immunosorbent Techniques,
pubmed-meshheading:12193540-Liver,
pubmed-meshheading:12193540-Liver Neoplasms, Experimental,
pubmed-meshheading:12193540-Male,
pubmed-meshheading:12193540-Methylcholanthrene,
pubmed-meshheading:12193540-Milk Proteins,
pubmed-meshheading:12193540-Phosphorylation,
pubmed-meshheading:12193540-Phosphotyrosine,
pubmed-meshheading:12193540-Rats,
pubmed-meshheading:12193540-Rats, Inbred F344,
pubmed-meshheading:12193540-STAT5 Transcription Factor,
pubmed-meshheading:12193540-Trans-Activators,
pubmed-meshheading:12193540-Transfection,
pubmed-meshheading:12193540-Tumor Cells, Cultured
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pubmed:year |
2002
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pubmed:articleTitle |
Stimulation of hepatic signal transducer and activator of transcription 5b by GH is not altered by 3-methylcholanthrene.
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pubmed:affiliation |
Department of Pharmacology, Medical Sciences Building, University of Toronto, Toronto, Ontario, Canada M5S 1A8.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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