Linked Life Data

12193122

Source:http://linkedlifedata.com/resource/pubmed/id/12193122

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2002-8-23
pubmed:abstractText
Hypertension is a major risk factor in the development of cardiovascular disease. Adenovirus gene transfer of endothelin-1 (Ad.CMV.ET-1) in rats produced significant (5-fold) increases in plasma ET-1 and systemic blood pressure (46%) 4 days after viral administration, compared with beta-galactosidase (Ad.CMV.beta-gal) injected as control. The density (B(max)) of the ET receptor ET(A) measured in aortas was reduced significantly by more than 50% to 17+/-2 fmol.mg(-1) of protein for the Ad.CMV.ET-1 group compared with 39+/-6 fmol x mg(-1) of protein for the control. There was no change in the density of the smaller population of the ET(B) sub-type. In agreement, the ratio of ET(A) mRNA to cyclophilin mRNA (a housekeeping gene) measured by Northern analysis was reduced in Ad.CMV.ET-1 rats compared with controls. The ratio of mRNA encoding the ET(B) sub-type did not change. ET-1 vasoconstriction was significantly reduced (P<0.05) in aortas from Ad.CMV.ET-1-treated rats [pD(2)=8.67+/-0.14 (where pD(2) is -log(10)EC(50)); n=11] versus the control (pD(2)=9.11+/-0.06; n=14) but there was no significant difference in the potency of two other vasoconstrictors tested (noradrenaline and Arg-vasopressin), indicating this was a specific effect on ET receptors. There was no change in the affinity of ET-1 binding to either receptor sub-type in the experimental group compared with the control, demonstrating that the attenuation in the constrictor response is the result of the reduced density of receptors rather than a change in affinity. The results show that ET(A) (but not ET(B)) receptors are modulated in this experimental model of hypertension and provide further evidence for selective blockade of the ET(A) receptor as a therapeutic strategy.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0143-5221
pubmed:author
pubmed:issnType
Print
pubmed:volume
103 Suppl 48
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
357S-362S
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12193122-Animals, pubmed-meshheading:12193122-Aorta, pubmed-meshheading:12193122-Arginine Vasopressin, pubmed-meshheading:12193122-Binding, Competitive, pubmed-meshheading:12193122-Blotting, Northern, pubmed-meshheading:12193122-Dose-Response Relationship, Drug, pubmed-meshheading:12193122-Endothelin-1, pubmed-meshheading:12193122-Hypertension, pubmed-meshheading:12193122-Male, pubmed-meshheading:12193122-Models, Animal, pubmed-meshheading:12193122-Muscle, Smooth, pubmed-meshheading:12193122-Muscle Contraction, pubmed-meshheading:12193122-Norepinephrine, pubmed-meshheading:12193122-RNA, Messenger, pubmed-meshheading:12193122-Rats, pubmed-meshheading:12193122-Rats, Wistar, pubmed-meshheading:12193122-Receptor, Endothelin A, pubmed-meshheading:12193122-Receptor, Endothelin B, pubmed-meshheading:12193122-Receptors, Endothelin, pubmed-meshheading:12193122-Vasoconstrictor Agents
pubmed:year
2002
pubmed:articleTitle
Elevated systemic levels of endothelin-1 and blood pressure correlate with blunted constrictor responses and downregulation of endothelin(A), but not endothelin(B), receptors in an animal model of hypertension.
pubmed:affiliation
Clinical Pharmacology Unit, University of Cambridge, Level 6, Centre for Clinical Investigation, Box 110, Addenbrooke's Hospital, Cambridge CB2 2QQ, U.K.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't