Source:http://linkedlifedata.com/resource/pubmed/id/12193100
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
2002-8-23
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| pubmed:abstractText |
Trypanosoma cruzi is the aetiological agent of Chagas' disease, an important cause of chronic cardiomyopathy. We previously demonstrated a role for endothelin-1 (ET-1) in the pathogenesis of chagasic heart disease. In order to explore further the significance of ET-1 in chagasic heart disease, we infected ET-1 (flox/flox); alpha-MHC-Cre(+) (ET-1KO) mice, in which the ET-1 gene has been deleted from cardiac myocytes, with 10(4) T. cruzi (Brazil strain) trypomastigotes. As controls, we used ET-1 (flox/flox);Cre(-) (FLOX) and C57BL/6x129sv (WT) mice. All mice survived and were evaluated 150-160 days post-infection. Cardiac magnetic resonance imaging revealed a significant increase in right ventricular internal diameter in all infected animals except ET-1KO mice (control WT, 1.6+/-0.10 mm; infected WT, 2.8+/-0.15 mm; control FLOX, 2.04+/-0.02 mm; infected FLOX, 2.76+/-0.28 mm). There was no significant difference in right ventricular internal diameter between infected and uninfected ET-1KO mice (control ET-1KO, 1.83+/-0.11 mm; infected ET-1KO, 2.14+/-0.20 mm). In another series of experiments, transthoracic echocardiography was performed on uninfected as well as infected ET-1KO mice, and uninfected and infected FLOX mice. Both infected groups had an increased left ventricular end-diastolic diameter and reduced fractional shortening. In addition, relative wall thickness was also decreased in infected animals. However, the magnitude of these changes was less in infected ET-1KO mice. These data provide further support for a role for ET-1 in the pathogenesis of chronic chagasic heart disease, and indicate that cardiac myocytes are an important source of ET-1 in this disease.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
0143-5221
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| pubmed:author |
pubmed-author:ChandraMadhulikaM,
pubmed-author:FactorStephen MSM,
pubmed-author:HuangHuanH,
pubmed-author:JelicksLinda ALA,
pubmed-author:KisanukiYaz YYY,
pubmed-author:PestellRichard GRG,
pubmed-author:ShiraniJamshidJ,
pubmed-author:ShtutinVitaliyV,
pubmed-author:TanowitzHerbert BHB,
pubmed-author:WeissLouis MLM,
pubmed-author:WittnerMurrayM,
pubmed-author:YanagisawaMasashiM
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| pubmed:issnType |
Print
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| pubmed:volume |
103 Suppl 48
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
263S-266S
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:12193100-Animals,
pubmed-meshheading:12193100-Chagas Cardiomyopathy,
pubmed-meshheading:12193100-Echocardiography,
pubmed-meshheading:12193100-Endothelin-1,
pubmed-meshheading:12193100-Magnetic Resonance Imaging,
pubmed-meshheading:12193100-Male,
pubmed-meshheading:12193100-Mice,
pubmed-meshheading:12193100-Mice, Knockout,
pubmed-meshheading:12193100-Myocardium
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| pubmed:year |
2002
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| pubmed:articleTitle |
Role of cardiac myocyte-derived endothelin-1 in chagasic cardiomyopathy: molecular genetic evidence.
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| pubmed:affiliation |
Department of Pathology, Albert Einstein College of Medicine, 1300 Morris Park, Bronx, New York, NY 10461, U.S.A. huangh@aecom.yu.edu
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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