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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
2002-9-4
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pubmed:abstractText |
Type IIa Na/P(i) cotransporters are expressed in renal proximal brush border and are the major determinants of inorganic phosphate (P(i)) reabsorption. Their carboxyl-terminal tail contains information for apical expression, and interacts by means of its three terminal amino acids with several PSD95/DglA/ZO-1-like domain (PDZ)-containing proteins. Two of these proteins, NaPi-Cap1 and Na/H exchanger-regulatory factor 1 (NHERF1), colocalize with the cotransporter in the proximal brush border. We used opossum kidney cells to test the hypothesis of a potential role of PDZ-interactions on the apical expression of the cotransporter. We found that opossum kidney cells contain NaPi-Cap1 and NHERF1 mRNAs. For NHERF1, an apical location of the protein could be documented; this location probably reflects interaction with the cytoskeleton by means of the MERM-binding domain. Overexpression of PDZ domains involved in interaction with the cotransporter (PDZ-1/NHERF1 and PDZ-3/NaPi-Cap1) had a dominant-negative effect, disturbing the apical expression of the cotransporter without affecting the actin cytoskeleton or the basolateral expression of Na/K-ATPase. These data suggest an involvement of PDZ-interactions on the apical expression of type IIa cotransporters.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12192091-10027934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12192091-10069185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12192091-10079096,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/12192091-10748165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12192091-10821685,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/12192091-11713658,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/12192091-9691018
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
99
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11957-62
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12192091-Amino Acid Motifs,
pubmed-meshheading:12192091-Amino Acid Sequence,
pubmed-meshheading:12192091-Animals,
pubmed-meshheading:12192091-Blotting, Northern,
pubmed-meshheading:12192091-Blotting, Western,
pubmed-meshheading:12192091-Cell Line,
pubmed-meshheading:12192091-Phosphoproteins,
pubmed-meshheading:12192091-Protein Binding,
pubmed-meshheading:12192091-Sodium-Hydrogen Antiporter,
pubmed-meshheading:12192091-Sodium-Phosphate Cotransporter Proteins,
pubmed-meshheading:12192091-Symporters
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pubmed:year |
2002
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pubmed:articleTitle |
PDZ-domain interactions and apical expression of type IIa Na/P(i) cotransporters.
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pubmed:affiliation |
Institute of Physiology, University of Zurich, Wintherthurerstrasse 190, CH-8057 Zurich, Switzerland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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