Linked Life Data

12191492

Source:http://linkedlifedata.com/resource/pubmed/id/12191492

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2002-8-22
pubmed:abstractText
The proprotein convertase PC2 is primarily expressed in neuroendocrine cells where it mediates the proteolytic maturation of prohormones and proneuropeptides. We have identified in the upstream sequence of its gene a conserved domain partially homologous to the repressor element RE1/NRSE found in several genes for neuronal proteins. RE1/NRSE binds the silencing transcription factor REST/NRSF, a nuclear protein primarily found in nonneuronal cells. To determine the functionality of the PC2 gene RE1-like sequence (RE1-lk), we examined by electrophoretic mobility shift assays its ability to attach nuclear factors from PC2-expressing and nonexpressing cells. Specific binding factors were mostly detectable in PC2-non-expressing cells. These factors differ from REST/NRSF, as molar excess of competing RE1/NRSE could not prevent their binding to RE1-lk. Reciprocally, molar excess of RE1-lk could not prevent the binding of RE1/NRSE to the DNA-binding domain of a recombinant REST/NRSF. The presence of RE1-lk in cis reduced the ability of the PC2 promoter and the heterologous phosphoglycerate kinase promoter to drive expression of a green fluorescent protein reporter gene in transiently transfected PC2-nonexpressing cells, but not in PC2-expressing cells. These observations suggest that binding of transcription-silencing factors to the RE1-lk element may contribute to repression of the PC2 gene in nonneuroendocrine cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0169-328X
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
102
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
35-47
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:12191492-Amino Acid Motifs, pubmed-meshheading:12191492-Animals, pubmed-meshheading:12191492-Base Sequence, pubmed-meshheading:12191492-Cell Differentiation, pubmed-meshheading:12191492-Chromosome Mapping, pubmed-meshheading:12191492-DNA-Binding Proteins, pubmed-meshheading:12191492-Eukaryotic Cells, pubmed-meshheading:12191492-Gene Expression Regulation, Enzymologic, pubmed-meshheading:12191492-Gene Silencing, pubmed-meshheading:12191492-Genes, Regulator, pubmed-meshheading:12191492-Genes, Reporter, pubmed-meshheading:12191492-Hormones, pubmed-meshheading:12191492-Mice, pubmed-meshheading:12191492-Molecular Sequence Data, pubmed-meshheading:12191492-Neurosecretory Systems, pubmed-meshheading:12191492-Promoter Regions, Genetic, pubmed-meshheading:12191492-Proprotein Convertase 2, pubmed-meshheading:12191492-RNA, Messenger, pubmed-meshheading:12191492-Rats, pubmed-meshheading:12191492-Repressor Proteins, pubmed-meshheading:12191492-Subtilisins, pubmed-meshheading:12191492-Transcription Factors, pubmed-meshheading:12191492-Tumor Cells, Cultured
pubmed:year
2002
pubmed:articleTitle
Characterization of a repressor element in the promoter region of proprotein convertase 2 (PC2) gene.
pubmed:affiliation
Diseases of Aging Centre, Ottawa Health Research Institute, Ottawa Hospital, University of Ottawa, 725 Parkdale Avenue, Ottawa, Ontario K1Y 4K9, Canada. mmbikay@ohri.ca
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't