Source:http://linkedlifedata.com/resource/pubmed/id/12186826
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2002-8-20
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| pubmed:abstractText |
Cigarette smoking results in oxidative stress and inflammation in the lungs, which are involved in the pathogenesis of chronic obstructive pulmonary disease (COPD). 4-Hydroxy-2-nonenal (4-HNE), a highly reactive diffusible product of lipid peroxidation, is a key mediator of oxidant-induced cell signaling and apoptosis. 4-HNE has a high affinity toward cysteine, histidine, and lysine groups and forms direct protein adducts. We investigated the presence of 4-HNE-modified proteins in lung tissue obtained from subjects with and without COPD. We studied 23 current or ex-smokers with similar smoking histories with COPD (n = 11; FEV(1) < 70% predicted) or without COPD (n = 12; FEV(1) > 84% predicted) who had undergone lung resection. As 4-HNE and transforming growth factor-beta(1) (TGF-beta(1)) can modulate gamma-glutamylcysteine synthetase (gamma-GCS) mRNA levels in lung cells, we assessed the relations between 4-HNE-modified protein levels, FEV(1), gamma-GCS, and TGF-beta(1). 4-HNE-modified protein levels were elevated in airway and alveolar epithelial cells, endothelial cells, and neutrophils in subjects with COPD, compared with the levels in subjects without COPD (p < 0.01). We also observed a significant inverse correlation between the levels of 4-HNE adducts in alveolar epithelium, airway endothelium, and neutrophils and FEV(1) (p < 0.05) and a positive correlation between 4-HNE adducts and TGF-beta(1) protein and mRNA as well as gamma-GCS mRNA levels in airway and alveolar epithelium (p < 0.01). The elevated levels of 4-HNE may play a role in the signaling events in lung inflammation leading to the imbalance of the expression of both proinflammatory mediators and protective antioxidant genes in COPD.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
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| pubmed:issn |
1073-449X
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
15
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| pubmed:volume |
166
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
490-5
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12186826-Aldehydes,
pubmed-meshheading:12186826-Apoptosis,
pubmed-meshheading:12186826-Case-Control Studies,
pubmed-meshheading:12186826-Disease Progression,
pubmed-meshheading:12186826-Forced Expiratory Volume,
pubmed-meshheading:12186826-Humans,
pubmed-meshheading:12186826-Immunohistochemistry,
pubmed-meshheading:12186826-Inflammation,
pubmed-meshheading:12186826-Lipid Peroxidation,
pubmed-meshheading:12186826-Lung,
pubmed-meshheading:12186826-Macrophages, Alveolar,
pubmed-meshheading:12186826-Middle Aged,
pubmed-meshheading:12186826-Neutrophils,
pubmed-meshheading:12186826-Oxidative Stress,
pubmed-meshheading:12186826-Pulmonary Disease, Chronic Obstructive,
pubmed-meshheading:12186826-Severity of Illness Index,
pubmed-meshheading:12186826-Signal Transduction,
pubmed-meshheading:12186826-Smoking
|
| pubmed:year |
2002
|
| pubmed:articleTitle |
4-Hydroxy-2-nonenal, a specific lipid peroxidation product, is elevated in lungs of patients with chronic obstructive pulmonary disease.
|
| pubmed:affiliation |
Respiratory Medicine Unit, ELEGI Laboratory, University of Edinburgh Medical School, Wilkie Building, Teviot Place, Edinburgh EH8 9AG, Scotland, UK. irfan.rahman@ed.ac.uk
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|