Linked Life Data

12186781

Source:http://linkedlifedata.com/resource/pubmed/id/12186781

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-8-20
pubmed:abstractText
We have found that omega-hydroxy palmitic acid (16-hydroxy palmitic acid, omega-HPA) has both cell growth inhibiting and cell death inducing actions on human lung adenosquamous carcinoma cell line H596 and adenocarcinoma cell line A549. Further, these effects were dose- and time-dependent in both cell lines. However, in squamous carcinoma cell line H226, omega-HPA had no cytotoxic effect. On the other hand, in the human small cell lung carcinoma (SCLC) cell line H128, this compound showed weak cytotoxicity. The sensitivity toward omega-HPA was higher in H596 cells than in A549 cells. In both H596 and A549 cells, cell growth was inhibited to 24.4 and 9.4%, respectively, by treatment with 100 microM omega-HPA for 12 h. In the 24 h treatment cells, growth inhibition was increased to 100 and 38.1%, respectively. In cytotoxicity experiments, the number of dead cells increased with incubation times in the presence of omega-HPA: on three days incubation with 100 microM omega-HPA, viability was 0 and 13.5%, respectively, in H596 and A549 cells. Further, the fragmentation of DNA to oligonucleosomal-sized ladder fragments, which is an index of apoptosis, was observed in both cell lines on treatment with omega-HPA. Therefore, it is assumed that these cell deaths induced by omega-HPA, were apoptosis in these cell lines. Since the number of dead cells following treatment with omega-HPA decreased by treatment with omega-HPA in combination with Z-VAD-fmk, a caspase family inhibitor, it is thought that apoptotic cell death was related to caspase activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1025-8140
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
37-43
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12186781-Adenocarcinoma, pubmed-meshheading:12186781-Amino Acid Chloromethyl Ketones, pubmed-meshheading:12186781-Antineoplastic Agents, Phytogenic, pubmed-meshheading:12186781-Apoptosis, pubmed-meshheading:12186781-Carcinoma, pubmed-meshheading:12186781-Carcinoma, Adenosquamous, pubmed-meshheading:12186781-Carcinoma, Squamous Cell, pubmed-meshheading:12186781-Caspases, pubmed-meshheading:12186781-Cell Division, pubmed-meshheading:12186781-Cell Line, pubmed-meshheading:12186781-Cell Survival, pubmed-meshheading:12186781-Cysteine Proteinase Inhibitors, pubmed-meshheading:12186781-DNA, Neoplasm, pubmed-meshheading:12186781-DNA Fragmentation, pubmed-meshheading:12186781-Dose-Response Relationship, Drug, pubmed-meshheading:12186781-Humans, pubmed-meshheading:12186781-Lung Neoplasms, pubmed-meshheading:12186781-Palmitic Acids, pubmed-meshheading:12186781-Time Factors, pubmed-meshheading:12186781-Tumor Cells, Cultured
pubmed:year
2002
pubmed:articleTitle
The omega-hydroxy palmitic acid induced apoptosis in human lung carcinoma cell lines H596 and A549.
pubmed:affiliation
Department of Biochemistry, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Japan. aabe-bc@tokyo-med.ac.jp
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't