Linked Life Data

12184177

Source:http://linkedlifedata.com/resource/pubmed/id/12184177

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2002-8-19
pubmed:abstractText
A new method, based on liquid chromatography--electrospray/tandem mass spectrometry, for the determination of abscisic acid (ABA), an essential plant hormone that regulates key metabolic pathways and responses to environmental cues, has been developed. Substantial changes in extraction procedures are also proposed. Data indicate that the organic solvents classically used as extraction buffers can be substituted by an aqueous solution, resulting in the same amounts of extracted ABA. The new method, which uses minute amounts of plant tissue, has an estimated limit of detection below 50 pmol ABA/g, and the sensitivity of the technique allows the analysis of ABA in crude plant extracts. Overall, this new rapid, sensitive and accurate procedure to determine ABA will allow analysis of multiple samples in a short time and represents a clear advantage in comparison with the conventional procedures involving many preparative steps and large amounts of plant tissue.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0958-0344
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
228-34
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
Direct analysis of abscisic acid in crude plant extracts by liquid chromatography--electrospray/tandem mass spectrometry.
pubmed:affiliation
Department of Experimental Sciences, Universitat Jaume I, Campus Riu Sec, E-12071 Castelló, Spain. cadenas@exp.uji.es
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't