| pubmed-article:12183664 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12183664 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:12183664 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:12183664 | lifeskim:mentions | umls-concept:C0234562 | lld:lifeskim |
| pubmed-article:12183664 | lifeskim:mentions | umls-concept:C0036534 | lld:lifeskim |
| pubmed-article:12183664 | lifeskim:mentions | umls-concept:C0005528 | lld:lifeskim |
| pubmed-article:12183664 | lifeskim:mentions | umls-concept:C0443199 | lld:lifeskim |
| pubmed-article:12183664 | lifeskim:mentions | umls-concept:C0243071 | lld:lifeskim |
| pubmed-article:12183664 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:12183664 | pubmed:dateCreated | 2002-8-16 | lld:pubmed |
| pubmed-article:12183664 | pubmed:abstractText | Secretin is a gastrointestinal peptide belonging to the vasoactive intestinal peptide (VIP)/glucagon/pituitary adenylate cyclase-activating polypeptide (PACAP) family recently suggested to have therapeutic effects in autism. A direct effect on brain would require secretin to cross the blood-brain barrier (BBB), an ability other members of the VIP/PACAP family have. Herein, we examined whether a secretin analog (SA) radioactively labeled with (131)I (I-SA) could cross the BBB of 4-week-old mice. We found I-SA was rapidly cleared from serum with fragments not precipitating with acid appearing in brain and serum. Levels of radioactivity were corrected to reflect only intact I-SA as estimated by acid precipitation. After i.v. injection, I-SA was taken up by brain at a modest rate of 0.9 to 1.5 microl/g-mm. Capillary depletion, brain perfusion, and high-performance liquid chromatography were used to confirm the passage of intact I-SA across the BBB. I-SA entered every brain region, with the highest uptake into the hypothalamus and cerebrospinal fluid (CSF). Unlabeled SA (10 microg/mouse) did not inhibit uptake by brain but did inhibit clearance from blood and uptake by the CSF, colon, kidney, and liver. The decreased clearance of I-SA from blood increased the percentage of the i.v. injected dose taken up per brain (%Inj/g) from about 0.118 to 0.295%Inj/g. In conclusion, SA crosses the vascular barrier by a nonsaturable process and the choroid plexus by a saturable process in amounts that for other members of its family produce central nervous system (CNS) effects. This passage provides a pathway through which peripherally administered SA could affect the CNS. | lld:pubmed |
| pubmed-article:12183664 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12183664 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12183664 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12183664 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12183664 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12183664 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12183664 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12183664 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12183664 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:12183664 | pubmed:issn | 0022-3565 | lld:pubmed |
| pubmed-article:12183664 | pubmed:author | pubmed-author:BanksWilliam... | lld:pubmed |
| pubmed-article:12183664 | pubmed:author | pubmed-author:GouletMartinM | lld:pubmed |
| pubmed-article:12183664 | pubmed:author | pubmed-author:BoismenuRicha... | lld:pubmed |
| pubmed-article:12183664 | pubmed:author | pubmed-author:NiehoffMichae... | lld:pubmed |
| pubmed-article:12183664 | pubmed:author | pubmed-author:RuscheJames... | lld:pubmed |
| pubmed-article:12183664 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12183664 | pubmed:volume | 302 | lld:pubmed |
| pubmed-article:12183664 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12183664 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12183664 | pubmed:pagination | 1062-9 | lld:pubmed |
| pubmed-article:12183664 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:12183664 | pubmed:meshHeading | pubmed-meshheading:12183664... | lld:pubmed |
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| pubmed-article:12183664 | pubmed:meshHeading | pubmed-meshheading:12183664... | lld:pubmed |
| pubmed-article:12183664 | pubmed:meshHeading | pubmed-meshheading:12183664... | lld:pubmed |
| pubmed-article:12183664 | pubmed:meshHeading | pubmed-meshheading:12183664... | lld:pubmed |
| pubmed-article:12183664 | pubmed:meshHeading | pubmed-meshheading:12183664... | lld:pubmed |
| pubmed-article:12183664 | pubmed:meshHeading | pubmed-meshheading:12183664... | lld:pubmed |
| pubmed-article:12183664 | pubmed:meshHeading | pubmed-meshheading:12183664... | lld:pubmed |
| pubmed-article:12183664 | pubmed:meshHeading | pubmed-meshheading:12183664... | lld:pubmed |
| pubmed-article:12183664 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12183664 | pubmed:articleTitle | Differential transport of a secretin analog across the blood-brain and blood-cerebrospinal fluid barriers of the mouse. | lld:pubmed |
| pubmed-article:12183664 | pubmed:affiliation | Geriatric Research, Education, and Clinical Center, St. Louis University School of Medicine, 915 N. Grand Boulevard, St. Louis, MO 63106, USA. bankswa@slu.edu | lld:pubmed |
| pubmed-article:12183664 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12183664 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:12183664 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:12183664 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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