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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2002-8-15
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pubmed:abstractText |
Lens epithelium-derived growth factor p75 (LEDGF/p75) is a nuclear autoantigen in atopic disorders implicated in cellular protection against stress-induced apoptosis. We observed that LEDGF/p75 was cleaved during apoptosis into fragments of 65 and 58 kD generated by caspases-3 and -7 cleaving at three sites: DEVPD30/G, DAQD486/G and WEID85/N. Sequence analysis revealed that the DEVPD30/G and WEID85/N sites lie within the highly conserved HATH (homologous to amino terminus of hepatoma-derived growth factor) region, also known as PWWP domain. Alignment of proteins containing this domain failed to reveal conservation of the DEVPD30/G and WEID85/N sites, suggesting that the HATH/PWWP domain of LEDGF/p75 may be specifically targeted by caspases. Overexpression of LEDGF/p75 protected HepG2 cells from serum starvation-induced cell death, whereas expression of the 65 kD fragment failed to protect. The apoptotic cleavage of LEDGF/p75 may contribute to the pathogenesis of atopic disorders by abrogating its pro-survival function and enhancing its immunogenicity.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CASP3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CASP7 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 7,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Serum-Free,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Hydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/lens epithelium-derived growth...
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1350-9047
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
915-25
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:12181742-Apoptosis,
pubmed-meshheading:12181742-Asthma,
pubmed-meshheading:12181742-Autoimmune Diseases,
pubmed-meshheading:12181742-Binding Sites,
pubmed-meshheading:12181742-Caspase 3,
pubmed-meshheading:12181742-Caspase 7,
pubmed-meshheading:12181742-Caspases,
pubmed-meshheading:12181742-Cell Survival,
pubmed-meshheading:12181742-Culture Media, Serum-Free,
pubmed-meshheading:12181742-Dermatitis, Atopic,
pubmed-meshheading:12181742-Gene Expression Regulation,
pubmed-meshheading:12181742-HeLa Cells,
pubmed-meshheading:12181742-Humans,
pubmed-meshheading:12181742-Hypersensitivity, Immediate,
pubmed-meshheading:12181742-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:12181742-Jurkat Cells,
pubmed-meshheading:12181742-Molecular Sequence Data,
pubmed-meshheading:12181742-Mutation,
pubmed-meshheading:12181742-Peptide Fragments,
pubmed-meshheading:12181742-Peptide Hydrolases,
pubmed-meshheading:12181742-Rhinitis,
pubmed-meshheading:12181742-Sequence Homology, Amino Acid,
pubmed-meshheading:12181742-Sequence Homology, Nucleic Acid
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pubmed:year |
2002
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pubmed:articleTitle |
Caspase cleavage of the nuclear autoantigen LEDGF/p75 abrogates its pro-survival function: implications for autoimmunity in atopic disorders.
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pubmed:affiliation |
Department of Biochemistry and Microbiology, Center for Molecular Biology and Gene Therapy, Loma Linda University School of Medicine, Loma Linda, CA 92354, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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