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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2002-8-15
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pubmed:abstractText |
This study was undertaken to demonstrate the role of the RhoA/Rho kinase pathway in endothelin-1 (ET-1)-induced contraction of the rabbit basilar artery. Isometric tension and Western blot were used to examine ET-1-induced contraction and RhoA activation. The upstream effect on ET-1-induced RhoA activity was determined by using ET(A) and ET(B) receptor antagonists, protein kinase C (PKC), tyrosine kinase, and phosphatidylinositol-3 kinase inhibitors. The downstream effect of ET-1-induced contraction and RhoA activity was studied in the presence of the Rho kinase inhibitor Y-27632. The effect of Rho kinase inhibitor on ET-1-induced myosin light chain (MLC) phosphorylation was investigated by using urea-glycerol-PAGE immunoblotting. We found 1) ET-1 increased RhoA activity (membrane binding RhoA) in a concentration-dependent manner; 2) ET(A), but not ET(B), receptor antagonist abolished the effect of ET-1 on RhoA activation; 3) phosphodylinositol-3 kinase inhibitor, but not PKC and tyrosine kinase inhibitors, reduced ET-1-induced RhoA activation; 4) Rho kinase inhibitor Y-27632 (10 microM) inhibited ET-1-induced contraction; and 5) ET-1 increased the level of MLC phosphorylation. Rho kinase inhibitor Y-27632 reduced the effect of ET-1 on MLC phosphorylation. This study demonstrated that RhoA/Rho kinase activation is involved in ET-1-induced contraction in the rabbit basilar artery. Phosphodylinositol-3 kinase and MLC might be the upstream and downstream factors of RhoA activation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antihypertensive Agents,
http://linkedlifedata.com/resource/pubmed/chemical/BQ 610,
http://linkedlifedata.com/resource/pubmed/chemical/BQ 788,
http://linkedlifedata.com/resource/pubmed/chemical/Endothelin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Piperidines,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Endothelin A,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Endothelin B,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Endothelin,
http://linkedlifedata.com/resource/pubmed/chemical/rho-Associated Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/rhoA GTP-Binding Protein
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0363-6135
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
283
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
H983-9
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:12181127-Animals,
pubmed-meshheading:12181127-Antihypertensive Agents,
pubmed-meshheading:12181127-Basilar Artery,
pubmed-meshheading:12181127-Endothelin-1,
pubmed-meshheading:12181127-Enzyme Activation,
pubmed-meshheading:12181127-Enzyme Inhibitors,
pubmed-meshheading:12181127-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:12181127-Male,
pubmed-meshheading:12181127-Oligopeptides,
pubmed-meshheading:12181127-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:12181127-Piperidines,
pubmed-meshheading:12181127-Protein Kinase C,
pubmed-meshheading:12181127-Protein-Serine-Threonine Kinases,
pubmed-meshheading:12181127-Protein-Tyrosine Kinases,
pubmed-meshheading:12181127-Rabbits,
pubmed-meshheading:12181127-Receptor, Endothelin A,
pubmed-meshheading:12181127-Receptor, Endothelin B,
pubmed-meshheading:12181127-Receptors, Endothelin,
pubmed-meshheading:12181127-Vasoconstriction,
pubmed-meshheading:12181127-Vasospasm, Intracranial,
pubmed-meshheading:12181127-rho-Associated Kinases,
pubmed-meshheading:12181127-rhoA GTP-Binding Protein
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pubmed:year |
2002
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pubmed:articleTitle |
Mechanism of RhoA/Rho kinase activation in endothelin-1- induced contraction in rabbit basilar artery.
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pubmed:affiliation |
Department of Neurosurgery, University of Mississippi Medical Center, Jackson, Mississippi 39216, USA.
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pubmed:publicationType |
Journal Article
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