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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2002-8-14
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pubmed:abstractText |
An 18 A resolution structure of the 12-subunit yeast RNA polymerase II (RNAPII) calculated from electron microscope images of single particles preserved in amorphous ice reveals the conformation of the enzyme in solution. The Rpb4/Rpb7 polymerase subunit complex was localized and found to be ideally positioned to determine the path of the nascent RNA transcript. The RNAPII structure suggests a revised mode of interaction with promoter DNA and demonstrates that regulation of RNAPII must involve structural changes that render the enzyme competent for initiation.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0969-2126
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1117-25
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12176389-Binding Sites,
pubmed-meshheading:12176389-Crystallography, X-Ray,
pubmed-meshheading:12176389-DNA,
pubmed-meshheading:12176389-DNA, Fungal,
pubmed-meshheading:12176389-Humans,
pubmed-meshheading:12176389-Models, Molecular,
pubmed-meshheading:12176389-Multienzyme Complexes,
pubmed-meshheading:12176389-Promoter Regions, Genetic,
pubmed-meshheading:12176389-Protein Subunits,
pubmed-meshheading:12176389-RNA,
pubmed-meshheading:12176389-RNA Polymerase II,
pubmed-meshheading:12176389-Yeasts
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pubmed:year |
2002
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pubmed:articleTitle |
Structure of yeast RNA polymerase II in solution: implications for enzyme regulation and interaction with promoter DNA.
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pubmed:affiliation |
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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