12176335

Source:http://linkedlifedata.com/resource/pubmed/id/12176335

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0043393, umls-concept:C0086860, umls-concept:C0162326, umls-concept:C0271510, umls-concept:C0678594, umls-concept:C1336572
pubmed:issue	14
pubmed:dateCreated	2002-8-14
pubmed:abstractText	The general transcription factor TFIID is composed of the TATA box binding protein (TBP) and multiple TBP-associated factors (TAFs). In yeast, promoters can be grouped into two classes based on the involvement of TAFs. TAF-dependent (TAF(dep)) promoters require TAFs for transcription, and TBP and TAFs are present at comparable levels on these promoters. TAF-independent (TAF(ind)) promoters do not require TAFs for activity, and TAFs are either absent or present at levels far below those of TBP on these promoters. Here, we demonstrate that the upstream activating sequence (UAS) mediates the selective recruitment of TAFs to TAF(dep) promoters. A TAF(ind) UAS fails to recruit TAFs and to direct efficient transcription when inserted upstream of a TAF(dep) core promoter. This transcriptional defect can be overcome by a potent activator, indicating that a strong activation domain can compensate for the absence of TAFs on a TAF(dep) core promoter. Our results reveal a requirement for compatibility between the UAS and core promoter and thus help explain previous reports that only certain yeast UAS-core promoter combinations and mammalian enhancer-promoter combinations are efficiently transcribed. The differential recruitment of TAFs by UASs provides strong evidence for the proposal that in vivo TAFs are the targets of some, but not all, activators.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/9107782
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/TATA-Box Binding Protein
pubmed:status	MEDLINE
pubmed:month	Jul
pubmed:issn	0960-9822
pubmed:author	pubmed-author:BhaumikSukesh RSR, pubmed-author:DixitBharat LBL, pubmed-author:GreenMichael RMR, pubmed-author:LiLeiL, pubmed-author:LiXiao-YongXY, pubmed-author:ShenWu-ChengWC, pubmed-author:ZhuXiaocunX
pubmed:issnType	Print
pubmed:day	23
pubmed:volume	12
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	1240-4
pubmed:dateRevised	2008-11-21
pubmed:meshHeading	pubmed-meshheading:12176335-Promoter Regions, Genetic, pubmed-meshheading:12176335-Saccharomyces cerevisiae, pubmed-meshheading:12176335-TATA-Box Binding Protein
pubmed:year	2002
pubmed:articleTitle	Selective recruitment of TAFs by yeast upstream activating sequences. Implications for eukaryotic promoter structure.
pubmed:affiliation	Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester 01605, USA.
pubmed:publicationType	Journal Article, Research Support, U.S. Gov't, P.H.S.