Source:http://linkedlifedata.com/resource/pubmed/id/12175012
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2002-8-14
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| pubmed:databankReference | |
| pubmed:abstractText |
The T locus of soybean (Glycine max (L.) Merr.) controls pubescence and seed coat color and is presumed to encode flavonoid 3'-hydroxylase (F3'H). The dominant T and the recessive t allele of the locus produce brown and gray pubescence, respectively. PCR primers were constructed based on the sequence of a soybean EST clone homologous to the F3'H gene. A putative full-length cDNA, sf3'h1 was isolated by 3' and 5' RACE. Sequence analysis revealed that sf3'h1 consists of 1690 nucleotides encoding 513 amino acids. It had 68% and 66% homology with corresponding F3'H protein sequences of petunia and Arabidopsis, respectively. A conserved amino acid sequence of F3'H proteins, GGEK, was found in the deduced polypeptide. Sequence analysis of the gene from a pair of near-isogenic lines for T, To7B (TT, brown) and To7G (tt, gray) revealed that they differed by a single C deletion in the coding region of To7G. The deletion changed the subsequent reading frame resulting in a truncated polypeptide lacking the GGEK consensus sequence and the heme-binding domain. Genomic Southern analysis probed by sf3'h1 revealed restriction fragment length polymorphisms between cultivars with different pubescence color. Further, sf3'h1 was mapped at the same position with T locus on LG3(c2). PCR-RFLP analysis was performed to detect the single-base deletion. To7B and three cultivars with brown pubescence exhibited shorter fragments, while To7G and three cultivars with gray pubescence had longer fragments due to the single-base deletion. The PCR-RFLP marker co-segregated with genotypes at the T locus in a F2 population segregating for the T locus. The above results strongly suggest that sJ3'h1 represents the T gene of soybean responsible for pubescence color and that the single-base deletion may be responsible for gray pubescence color.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Plant,
http://linkedlifedata.com/resource/pubmed/chemical/Mixed Function Oxygenases,
http://linkedlifedata.com/resource/pubmed/chemical/Pigments, Biological,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Plant,
http://linkedlifedata.com/resource/pubmed/chemical/flavonoid 3'-hydroxylase
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| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
0167-4412
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
50
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
187-96
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| pubmed:dateRevised |
2005-11-17
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| pubmed:meshHeading |
pubmed-meshheading:12175012-Alleles,
pubmed-meshheading:12175012-Amino Acid Sequence,
pubmed-meshheading:12175012-Base Sequence,
pubmed-meshheading:12175012-Chromosome Mapping,
pubmed-meshheading:12175012-Cloning, Molecular,
pubmed-meshheading:12175012-Crosses, Genetic,
pubmed-meshheading:12175012-Cytochrome P-450 Enzyme System,
pubmed-meshheading:12175012-DNA, Complementary,
pubmed-meshheading:12175012-DNA, Plant,
pubmed-meshheading:12175012-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:12175012-Gene Expression Regulation, Plant,
pubmed-meshheading:12175012-Genes, Recessive,
pubmed-meshheading:12175012-Genotype,
pubmed-meshheading:12175012-Mixed Function Oxygenases,
pubmed-meshheading:12175012-Molecular Sequence Data,
pubmed-meshheading:12175012-Mutation,
pubmed-meshheading:12175012-Pigments, Biological,
pubmed-meshheading:12175012-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:12175012-RNA, Plant,
pubmed-meshheading:12175012-Sequence Alignment,
pubmed-meshheading:12175012-Sequence Analysis, DNA,
pubmed-meshheading:12175012-Sequence Deletion,
pubmed-meshheading:12175012-Sequence Homology, Amino Acid,
pubmed-meshheading:12175012-Sequence Homology, Nucleic Acid,
pubmed-meshheading:12175012-Soybeans
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| pubmed:year |
2002
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| pubmed:articleTitle |
A single-base deletion in soybean flavonoid 3'-hydroxylase gene is associated with gray pubescence color.
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| pubmed:affiliation |
National Institute of Crop Science, Tsukuba, Ibaraki, Japan.
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| pubmed:publicationType |
Journal Article
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