Linked Life Data

12172968

Source:http://linkedlifedata.com/resource/pubmed/id/12172968

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2002-8-12
pubmed:abstractText
Aspergillus fumigatus is an important opportunistic human pathogenic fungus. In severely immunocompromised patients, the fungus causes life-threatening diseases, such as pneumonia and invasive aspergillosis. In order to obtain a better understanding of the key elements involved in A. fumigatus virulence and for identifying possible drug targets, it is essential to be able to generate gene-deletion strains. Until recently, the molecular techniques available did not provide a rapid method for gene deletion. A novel method described for A. nidulans was adapted for A. fumigatus. This method is quick and produces an increased homologous recombination efficiency. By using an Escherichia coli strain expressing the lambda red operon, it is possible to induce an in vivo recombination of a PCR fragment flanked by >50-bp regions with a cosmid containing the gene of interest. This produces cosmids in which the gene of interest has been replaced by a bi-functional marker. Such cosmids have large flanking regions surrounding the selectable marker pyrG of A. fumigatus used here, which result in high recombination efficiencies in A. fumigatus. Here, we identified a new ABC transporter-encoding gene in A. fumigatus, designated abcA. By using this method, an A. fumigatus knock-out mutant was generated, providing evidence that this method of generating gene deletions can also be used in A. fumigatus and significantly broadens our repertoire of molecular techniques to study A. fumigatus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0172-8083
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
268-74
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
A novel method used to delete a new Aspergillus fumigatus ABC transporter-encoding gene.
pubmed:affiliation
Institut für Mikrobiologie, Universität Hannover, Am Schneiderberg 50, 30167 Hannover, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't