Linked Life Data

12170182

Source:http://linkedlifedata.com/resource/pubmed/id/12170182

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2002-8-9
pubmed:abstractText
In a retrospective study we analysed the levels of the DNA repair protein O(6)-methylguanine-DNA methyltransferase (MGMT) in melanoma metastases in patients receiving dacarbazine (DTIC) either as a single drug or as part of combination chemotherapy regimens, and related the expression levels to the clinical response to treatment. Biopsies of subcutaneous and lymph node metastases obtained before chemotherapy in 65 patients with disseminated malignant melanoma were examined for MGMT protein levels by immunohistochemistry using a monoclonal anti-human MGMT antibody. All patients received chemotherapy with DTIC, given either as a single drug or in combination with vindesine and in some cases cisplatin. DTIC as single agent was given to 44 patients, while 21 received combination chemotherapy. Objective responses to chemotherapy were seen in 12 patients, while 53 patients failed to respond to treatment. The expression of MGMT was determined according to the proportion of antibody-stained tumour cells, using a cut-off level of 50%. In 12 of the patients more than one metastasis was analysed, and in seven of these cases the MGMT expression differed between tumours in the same individual. Among the responders a larger proportion (six out of 12, 50%) had tumours containing less than 50% MGMT-positive tumour cells than among the non-responders (12 out of 53, 23%). These data are consistent with the hypothesis that MGMT contributes to resistance to DTIC-based treatment, although the difference between responders and non-responders with respect to the proportion of MGMT-positive tumour cells was not statistically significant (P = 0.077).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0960-8931
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
335-42
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12170182-Adult, pubmed-meshheading:12170182-Aged, pubmed-meshheading:12170182-Antibodies, Monoclonal, pubmed-meshheading:12170182-Antineoplastic Agents, Alkylating, pubmed-meshheading:12170182-Antineoplastic Combined Chemotherapy Protocols, pubmed-meshheading:12170182-Cisplatin, pubmed-meshheading:12170182-DNA, Neoplasm, pubmed-meshheading:12170182-DNA Damage, pubmed-meshheading:12170182-DNA Methylation, pubmed-meshheading:12170182-DNA Repair, pubmed-meshheading:12170182-Dacarbazine, pubmed-meshheading:12170182-Drug Resistance, Neoplasm, pubmed-meshheading:12170182-Female, pubmed-meshheading:12170182-Humans, pubmed-meshheading:12170182-Immunoenzyme Techniques, pubmed-meshheading:12170182-Lymphatic Metastasis, pubmed-meshheading:12170182-Male, pubmed-meshheading:12170182-Melanoma, pubmed-meshheading:12170182-Middle Aged, pubmed-meshheading:12170182-Neoplasm Proteins, pubmed-meshheading:12170182-O(6)-Methylguanine-DNA Methyltransferase, pubmed-meshheading:12170182-Observer Variation, pubmed-meshheading:12170182-Prodrugs, pubmed-meshheading:12170182-Vindesine
pubmed:year
2002
pubmed:articleTitle
Analysis of O(6)-methylguanine-DNA methyltransferase in melanoma tumours in patients treated with dacarbazine-based chemotherapy.
pubmed:affiliation
Department of Oncology/Pathology, Radiumhemmet, Karolinska Hospital, S-171 76 Stockholm, Sweden.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't, Evaluation Studies