12169592

Source:http://linkedlifedata.com/resource/pubmed/id/12169592

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0017428, umls-concept:C0022130, umls-concept:C0283807, umls-concept:C0370215, umls-concept:C0376624, umls-concept:C0600414, umls-concept:C0679058, umls-concept:C0683598, umls-concept:C1547699, umls-concept:C2700640, umls-concept:C2827666
pubmed:issue	17
pubmed:dateCreated	2002-8-9
pubmed:abstractText	Strains of Escherichia coli causing enterohemorrhagic colitis belonging to the O157:H7 lineage are reported to be highly related. Fifteen strains of E. coli O157:H7 and 1 strain of E. coli O46:H(-) (nonflagellated) were examined for the presence of potassium tellurite resistance (Te(r)). Te(r) genes comprising terABCDEF were shown previously to be part of a pathogenicity island also containing integrase, phage, and urease genes. PCR analysis, both conventional and light cycler based, demonstrated that about one-half of the Te(r) E. coli O157:H7 strains (6 of 15), including the Sakai strain, which has been sequenced, carried a single copy of the Te(r) genes. Five of the strains, including EDL933, which has also been sequenced, contained two copies. Three other O157:H7 strains and the O46:H(-) strain did not contain the Te(r) genes. In strains containing two copies, the Te(r) genes were associated with the serW and serX tRNA genes. Five O157:H7 strains resembled the O157 Sakai strain whose sequence contained one copy, close to serX, whereas in one isolate the single copy was associated with serW. There was no correlation between Te(r) and the ability to produce Shiga toxin ST1 or ST2. The Te(r) MIC for most strains, containing either one or two copies, was 1,024 micro g/ml, although for a few the MIC was intermediate, 64 to 128 micro g/ml, which could be increased to 512 micro g/ml by pregrowth of strains in subinhibitory concentrations of potassium tellurite. Reverse transcriptase PCR analysis confirmed that in most strains Te(r) was constitutive but that in the rest it was inducible and involved induction of terB and terC genes. Only the terB, -C, -D, and -E genes are required for Te(r). The considerable degree of homology between the ter genes on IncH12 plasmid R478, which originated in Serratia marcescens, and pTE53, from an E. coli clinical isolate, suggests that the pathogenicity island was acquired from a plasmid. This work demonstrates diversity among E. coli O157:H7 isolates, at least as far as the presence of Te(r) genes is concerned.
pubmed:grant	http://linkedlifedata.com/resource/pubmed/grant/AI 44387
pubmed:commentsCorrections	http://linkedlifedata.com/resource/pubmed/commentcorrection/12169592-10069007, http://linkedlifedata.com/resource/pubmed/commentcorrection/12169592-10203839, http://linkedlifedata.com/resource/pubmed/commentcorrection/12169592-10655350, http://linkedlifedata.com/resource/pubmed/commentcorrection/12169592-10678953, http://linkedlifedata.com/resource/pubmed/commentcorrection/12169592-11016400, http://linkedlifedata.com/resource/pubmed/commentcorrection/12169592-11064204, http://linkedlifedata.com/resource/pubmed/commentcorrection/12169592-11206551, http://linkedlifedata.com/resource/pubmed/commentcorrection/12169592-11238985, http://linkedlifedata.com/resource/pubmed/commentcorrection/12169592-11698378, http://linkedlifedata.com/resource/pubmed/commentcorrection/12169592-1741460, http://linkedlifedata.com/resource/pubmed/commentcorrection/12169592-2987187, http://linkedlifedata.com/resource/pubmed/commentcorrection/12169592-374351, http://linkedlifedata.com/resource/pubmed/commentcorrection/12169592-3892231, http://linkedlifedata.com/resource/pubmed/commentcorrection/12169592-401494, http://linkedlifedata.com/resource/pubmed/commentcorrection/12169592-7665479, http://linkedlifedata.com/resource/pubmed/commentcorrection/12169592-7727633, http://linkedlifedata.com/resource/pubmed/commentcorrection/12169592-8345511, http://linkedlifedata.com/resource/pubmed/commentcorrection/12169592-8953703, http://linkedlifedata.com/resource/pubmed/commentcorrection/12169592-8981981, http://linkedlifedata.com/resource/pubmed/commentcorrection/12169592-9278503, http://linkedlifedata.com/resource/pubmed/commentcorrection/12169592-9607864, http://linkedlifedata.com/resource/pubmed/commentcorrection/12169592-9665978
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/2985120R
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Shiga Toxin, http://linkedlifedata.com/resource/pubmed/chemical/Tellurium, http://linkedlifedata.com/resource/pubmed/chemical/tellurous acid
pubmed:status	MEDLINE
pubmed:month	Sep
pubmed:issn	0021-9193
pubmed:author	pubmed-author:BlattnerFredrick RFR, pubmed-author:BurlandN T ValerieNT, pubmed-author:KeelanMonikaM, pubmed-author:MartinIreneI, pubmed-author:NgLai-KingLK, pubmed-author:PernaNicole TNT, pubmed-author:RookerMichelleM, pubmed-author:TaylorDiane EDE
pubmed:issnType	Print
pubmed:volume	184
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	4690-8
pubmed:dateRevised	2009-11-18
pubmed:meshHeading	pubmed-meshheading:12169592-Drug Resistance, Bacterial, pubmed-meshheading:12169592-Escherichia coli O157, pubmed-meshheading:12169592-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12169592-Shiga Toxin, pubmed-meshheading:12169592-Tellurium
pubmed:year	2002
pubmed:articleTitle	Genomic variability of O islands encoding tellurite resistance in enterohemorrhagic Escherichia coli O157:H7 isolates.
pubmed:affiliation	Department of Medical Microbiology and Immunology, 1-28 Medical Sciences Building, University of Alberta, Edmonton, Alberta T6G 2H7, Canada. Diane.Taylor@ualberta.ca
pubmed:publicationType	Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't