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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2002-8-9
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pubmed:abstractText |
Human myxoid liposarcoma contains a characteristic t(12;16) chromosomal translocation that results in fusion of the N-terminal domain of the translocated in liposarcoma (TLS) protein to the C/EBP homologous protein (CHOP). TLS possesses structural motifs that suggest it may participate in RNA processing. We demonstrate that in human myxoid liposarcoma cells, wild-type TLS binds to RNA polymerase II (Pol II) via its N-terminal domain and to the transcription and translation factor Y-box binding protein-1 (YB-1) through its C-terminal domain. The liposarcoma fusion protein TLS/CHOP retains the ability to bind RNA Pol II but lacks the ability to recruit YB-1 due to replacement of the C-terminal domain of TLS by CHOP. In an in vivo splicing assay, YB-1 promotes splicing of adenovirus EIA pre-mRNA predominantly to the 13S isoform. The oncogenic TLS/CHOP fusion protein inhibits this splicing function of YB-1 in a dominant negative manner. When considered in conjunction with studies on other sarcoma fusion proteins, these data suggest that aberrant RNA splicing may be a common feature of human sarcomas.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenovirus E1A Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Heterogeneous-Nuclear...,
http://linkedlifedata.com/resource/pubmed/chemical/NFI Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins, Fusion,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Polymerase II,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Protein FUS,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/TLS-CHOP fusion protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor CHOP,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Y-Box-Binding Protein 1,
http://linkedlifedata.com/resource/pubmed/chemical/YBX1 protein, human
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0736-0266
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
723-9
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:12168660-Adenovirus E1A Proteins,
pubmed-meshheading:12168660-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:12168660-DNA-Binding Proteins,
pubmed-meshheading:12168660-Heterogeneous-Nuclear Ribonucleoproteins,
pubmed-meshheading:12168660-Humans,
pubmed-meshheading:12168660-Liposarcoma, Myxoid,
pubmed-meshheading:12168660-NFI Transcription Factors,
pubmed-meshheading:12168660-Nuclear Proteins,
pubmed-meshheading:12168660-Oncogene Proteins, Fusion,
pubmed-meshheading:12168660-RNA Polymerase II,
pubmed-meshheading:12168660-RNA Splicing,
pubmed-meshheading:12168660-RNA-Binding Protein FUS,
pubmed-meshheading:12168660-Ribonucleoproteins,
pubmed-meshheading:12168660-Transcription Factor CHOP,
pubmed-meshheading:12168660-Transcription Factors,
pubmed-meshheading:12168660-Tumor Cells, Cultured,
pubmed-meshheading:12168660-Y-Box-Binding Protein 1
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pubmed:year |
2002
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pubmed:articleTitle |
RNA splicing mediated by YB-1 is inhibited by TLS/CHOP in human myxoid liposarcoma cells.
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pubmed:affiliation |
Department of Orthopaedics and Sports Medicine, School of Medicine, University of Washington, Seattle 98108, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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