Linked Life Data

12167537

Source:http://linkedlifedata.com/resource/pubmed/id/12167537

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2002-8-8
pubmed:abstractText
The autolysin AtlC is the only known fibronectin-binding protein in Staphylococcus caprae strain 96007. The fibronectin-binding domain of AtlC consists of three repeats (AtlCR(1)R(2)R(3)), which are located between the two enzymatic domains. The AtlCR(1)R(2)R(3) domain and the AtlCR(1)R(2) and AtlCR(3) subdomains were expressed separately as His(6)-tagged proteins. In Western affinity blots, only AtlCR(1)R(2)R(3) and AtlCR(3) but not AtlCR(1)R(2) appeared to recognise fibronectin; however, in ELISA and Biacore experiments, all three bound fibronectin. The interaction between AtlCR(1)R(2)R(3) and fibronectin is multivalent and involves high- and low-affinity sites that are present in a 2:1 ratio. These distinct classes of binding sites may be situated on either or on both ligands.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0378-1097
pubmed:author
pubmed:issnType
Print
pubmed:day
6
pubmed:volume
213
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
193-7
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Several regions of the repeat domain of the Staphylococcus caprae autolysin, AtlC, are involved in fibronectin binding.
pubmed:affiliation
Staphylococci Unit, National Reference Center for Staphylococci, Institut Pasteur, 28 rue du Dr. Roux, 75724 Paris Cedex 15, France.
pubmed:publicationType
Journal Article