Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2002-8-7
pubmed:abstractText
Secretory Igs provide the first line of adaptive immune defense against ingested, inhaled, and sexually transmitted pathogens at mucosal surfaces. The polymeric Ig receptor regulates transport of dimeric IgA and pentameric IgM into external secretions. The level of expression of polymeric Ig receptor is controlled to a large extent by transcription of the PIGR gene in mucosal epithelial cells. Here we present a detailed analysis of the promoter of the PIGR gene by transient transfection of luciferase reporter plasmids into cultured cell lines. Comparisons of the human and mouse PIGR promoters in human and mouse intestinal and liver cell lines demonstrated that the human PIGR promoter was 4- to 5-fold more active than the mouse PIGR promoter in all cell types, and that both the human and mouse PIGR promoters were more active in intestinal than in liver cell lines. Targeted deletions of 22-bp segments of the human PIGR promoter revealed that the region from nt -63 to -84 is crucial for basal transcription, and that two upstream regions can act as positive or negative regulators. Point mutations within the region from nt -63 to -84 demonstrated that an E box motif, which binds the basic helix-loop-helix protein upstream stimulatory factor, is required for PIGR promoter activity. Two additional regulatory motifs were identified in the proximal promoter region: a binding site for AP2, and an inverted repeat motif that binds an unidentified protein. These findings suggest that cooperative binding of multiple transcription factors regulates basal activity of the human PIGR promoter.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
169
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1912-21
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:12165516-Animals, pubmed-meshheading:12165516-Base Sequence, pubmed-meshheading:12165516-Caco-2 Cells, pubmed-meshheading:12165516-DNA, pubmed-meshheading:12165516-Gene Expression Regulation, pubmed-meshheading:12165516-Genes, Immunoglobulin, pubmed-meshheading:12165516-Humans, pubmed-meshheading:12165516-Intestines, pubmed-meshheading:12165516-Liver, pubmed-meshheading:12165516-Mice, pubmed-meshheading:12165516-Molecular Sequence Data, pubmed-meshheading:12165516-Nuclear Proteins, pubmed-meshheading:12165516-Point Mutation, pubmed-meshheading:12165516-Promoter Regions, Genetic, pubmed-meshheading:12165516-Protein Binding, pubmed-meshheading:12165516-Rats, pubmed-meshheading:12165516-Receptors, Polymeric Immunoglobulin, pubmed-meshheading:12165516-Sequence Homology, Nucleic Acid, pubmed-meshheading:12165516-Species Specificity, pubmed-meshheading:12165516-Tissue Distribution, pubmed-meshheading:12165516-Transcription, Genetic
pubmed:year
2002
pubmed:articleTitle
Transcriptional regulation of the human polymeric Ig receptor gene: analysis of basal promoter elements.
pubmed:affiliation
Department of Microbiology and Immunology, University of Kentucky, Lexington, KY 40536, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.