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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2002-8-7
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pubmed:abstractText |
Peroxisome proliferator-activated receptor gamma (PPARgamma) is a ligand dependent transcriptional factor known to be a regulator of adipogenesis. Recent studies have also shown that stimulation of PPARgamma inhibits the transcriptional activities of other nuclear factors and down-regulates proinflammatory cytokine synthesis in T cells and monocytes. We examined, in the present study, the functional significance of PPARgamma expressed in fibroblast-like synovial cells (FLS) isolated from patients with rheumatoid arthritis (RA). Incubation of FLS with a synthetic PPARgamma ligand, troglitazone, inhibited endogenous production of TNF-alpha, IL-6 and IL-8, as well as matrix metalloprotease-3 (MMP-3), without inducing apoptosis of the cells. The gelatinase activity of FLS culture media was also inhibited by troglitazone. Electrophoretic mobility shift assay (EMSA) showed a significant reduction in the DNA binding activity of NF-kappaB in troglitazone-treated FLS in response to TNF-alpha or IL-1beta. Moreover, long-term cultivation of FLS with troglitazone resulted in morphological changes with marked lipid accumulation in these cells. Our results show a negative regulatory function for PPARgamma on cytokine and MMP production together with inhibition of cytokine-mediated inflammatory responses in rheumatoid synovial cells. Our results also suggest that FLS could differentiate into adipocyte-like cells in the presence of proper stimulatory signals including PPARgamma.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-10102814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-10529898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-10542237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-10555040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-10615998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-10671476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-10753637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-10782811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-10903334,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-11014356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-11120820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-11135615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-11213361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-2180403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-2194461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-3358796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-7568232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-7768881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-7848304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-8001151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-8011280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-8521497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-8521498,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-8755634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-9422508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-9422509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12165097-9811891
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chromans,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Gelatinases,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-8,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 3,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/Thiazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Thiazolidinediones,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/troglitazone
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
129
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
379-84
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12165097-Adipocytes,
pubmed-meshheading:12165097-Arthritis, Rheumatoid,
pubmed-meshheading:12165097-Cell Differentiation,
pubmed-meshheading:12165097-Cells, Cultured,
pubmed-meshheading:12165097-Chromans,
pubmed-meshheading:12165097-DNA,
pubmed-meshheading:12165097-Fibroblasts,
pubmed-meshheading:12165097-Gelatinases,
pubmed-meshheading:12165097-Humans,
pubmed-meshheading:12165097-Interleukin-6,
pubmed-meshheading:12165097-Interleukin-8,
pubmed-meshheading:12165097-Ligands,
pubmed-meshheading:12165097-Matrix Metalloproteinase 3,
pubmed-meshheading:12165097-NF-kappa B,
pubmed-meshheading:12165097-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:12165097-Signal Transduction,
pubmed-meshheading:12165097-Synovial Membrane,
pubmed-meshheading:12165097-Thiazoles,
pubmed-meshheading:12165097-Thiazolidinediones,
pubmed-meshheading:12165097-Transcription Factors,
pubmed-meshheading:12165097-Tumor Necrosis Factor-alpha
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pubmed:year |
2002
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pubmed:articleTitle |
Functional changes in rheumatoid fibroblast-like synovial cells through activation of peroxisome proliferator-activated receptor gamma-mediated signalling pathway.
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pubmed:affiliation |
First Department of Internal Medicine, Nagasaki University School of Medicine, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan.
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pubmed:publicationType |
Journal Article,
In Vitro
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